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Purified anti-mouse CD14 Antibody

Pricing & Availability
Clone
M14-23 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Rat IgG2a, κ
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Product Citations
publications
M14-23_Purified_CD14_Antibody_FC_051315
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were stained with purified CD14 (clone M14-23) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-Rat IgG PE.
  • M14-23_Purified_CD14_Antibody_FC_051315
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were stained with purified CD14 (clone M14-23) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-Rat IgG PE.
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150101 25 µg 77 CHF
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150102 100 µg 136 CHF
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14 in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD14-expressing NRK-52E transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking.1 M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)
Product Citations
  1. Li TF, et al. 2017. J Control Release. 128:268. PubMed
RRID
AB_2565713 (BioLegend Cat. No. 150101)
AB_2565713 (BioLegend Cat. No. 150102)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53 - 55 kD.
Distribution

Macrophages, dendritic cells, kupffer cells, and hepatocytes.

Function
LPS receptor and clearance of Gram-negative pathogens.
Ligand/Receptor
LPS.
Cell Type
Macrophages, Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.

Gene ID
12475 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 1    Revision Date: 05.19.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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