- Clone
- M14-23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LPS receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
150103 | 50 µg | 94 CHF | ||||
150104 | 500 µg | 325 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14 in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD14-expressing NRK-52E transfected cells.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: blocking.1 M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.
-
Application References
(PubMed link indicates BioLegend citation) -
- Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)
- RRID
-
AB_2565714 (BioLegend Cat. No. 150103)
AB_2565714 (BioLegend Cat. No. 150104)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein, 53 - 55 kD.
- Distribution
-
Macrophages, dendritic cells, kupffer cells, and hepatocytes.
- Function
- LPS receptor and clearance of Gram-negative pathogens.
- Ligand/Receptor
- LPS.
- Cell Type
- Macrophages, Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089. - Gene ID
- 12475 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD14 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD14 | M14-23 | FC |
Ultra-LEAF™ Purified anti-mouse CD14 | M14-23 | FC,Block |
PE anti-mouse CD14 | M14-23 | FC |
Spark YG™ 593 anti-mouse CD14 | M14-23 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
Ultra-LEAF™ Purified anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
PE anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
Spark YG™ 593 anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w...
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