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Purified anti-mouse CD25 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
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Product Citations
publications
3C7_Purified_CD25_CyTOF_112113
C57BL/6 mouse splenocytes stained with145Nd-anti-CD4 (RM4-5) and 150Nd-anti-CD25 (3C7). Data provided by DVS Sciences.
  • 3C7_Purified_CD25_CyTOF_112113
    C57BL/6 mouse splenocytes stained with145Nd-anti-CD4 (RM4-5) and 150Nd-anti-CD25 (3C7). Data provided by DVS Sciences.
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101913 100 µg 118 CHF
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Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  2. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
  3. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  4. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  5. Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
  6. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  7. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  8. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  9. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  10. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  11. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  12. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  13. Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
  14. Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
  15. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  16. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  17. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  18. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  19. Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
  20. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID
AB_2562798 (BioLegend Cat. No. 101913)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 3    Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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