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PE anti-mouse CD25 Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
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Product Citations
publications
3C7_PE_080207
Con A-activated (3days) C57BL/6 mouse splenocytes stained with 3C7 PE
  • 3C7_PE_080207
    Con A-activated (3days) C57BL/6 mouse splenocytes stained with 3C7 PE
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Cat # Size Price Quantity Check Availability Save
101903 25 µg 86 CHF
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101904 100 µg 213 CHF
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Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Sugiura A, et al. 2022. Immunity. 55:65. PubMed
  3. Kim HI, et al. 2022. Molecules. 27: . PubMed
  4. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  5. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  6. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  7. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  8. Lua Y, et al. 2012. Mol Immunol. 52:229. PubMed
  9. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  10. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  11. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  12. Roy S, et al. 2020. Sci Rep. 10:10992. PubMed
  13. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  14. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  15. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  16. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  17. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  18. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  19. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  20. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  21. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  22. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  23. An J, et al. 2022. iScience. 25:103570. PubMed
  24. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  25. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  26. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  27. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  28. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  29. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  30. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  31. Toyama S, et al. 2021. Int J Mol Sci. 22:. PubMed
  32. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  33. Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed
  34. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
  35. Toro Acevedo CA, et al. 2017. Front Microbiol. 8:2188. PubMed
  36. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  37. Zhang X, et al. 2021. Front Pharmacol. 12:629513. PubMed
  38. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  39. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  40. Haque A, et al. 2020. Exp Neurol. 330:113315. PubMed
  41. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  42. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  43. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  44. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  45. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  46. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  47. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  48. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  49. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
  50. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_312847 (BioLegend Cat. No. 101903)
AB_312847 (BioLegend Cat. No. 101904)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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