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Purified anti-mouse CD49b (pan-NK cells) Antibody

Pricing & Availability
Clone
DX5 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
DX5_Pure_071608.jpg
C57BL/6 mouse splenocytes stained with DX5 purified, followed by anti-rat IgG FITC
  • DX5_Pure_071608.jpg
    C57BL/6 mouse splenocytes stained with DX5 purified, followed by anti-rat IgG FITC
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108901 50 µg 89 CHF
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108902 500 µg 252 CHF
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Description

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-propagated NK1.1+ cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Arase H, et al. 2001. J. Immunol. 167:1141. (FC)
  2. Sepulveda H, et al. 1999. J. Immunol. 163:1133.
  3. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  4. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  5. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
  7. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  8. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
  9. Busche A, et al. 2011. J. Immunol. 186:2918. PubMed
  10. Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed
  11. Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed
  12. Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed
  13. Honjo K, et al. 2012. PNAS. PubMed.
  14. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
Product Citations
  1. Bruce DW, et al. 2021. Blood Adv. 5:4578. PubMed
  2. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  3. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  4. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  5. Seitz S, et al. 2022. Br J Cancer. 126:1470. PubMed
  6. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  7. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  8. Oertelt S, et al. 2006. J Immunol. 177:1655. PubMed
  9. Wu L, et al. 2022. Front Immunol. 13:860761. PubMed
  10. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  11. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  12. Si Y, et al. 2020. Sci Adv. 6:eaaw7713. PubMed
  13. Blume J, et al. 2019. Eur J Immunol. 49:121. PubMed
  14. Jogdand GM, et al. 2018. Front Immunol. 9:1041. PubMed
  15. Kim O, et al. 2017. Nat Commun. 10.1038/s41467-017-00729-8. PubMed
  16. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  17. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  18. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  19. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
  20. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  21. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  22. Blriot C, et al. 2021. Immunity. :. PubMed
  23. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  24. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  25. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  27. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  28. Gillory L, et al. 2013. PLoS One. 8:77753. PubMed
  29. Busche A, et al. 2011. J Immunol. 186:2918. PubMed
  30. Mizuno M, et al. 2021. Cancers (Basel). 13:. PubMed
  31. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  32. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  33. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  34. Chen X, et al. 2012. Endocrinology. 153:4622. PubMed
  35. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  36. Huang H, et al. 2013. Biomaterials. 34:10151. PubMed
  37. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  38. Seehus CR, et al. 2017. Nat Commun. 8:1900. PubMed
RRID
AB_313408 (BioLegend Cat. No. 108901)
AB_313408 (BioLegend Cat. No. 108902)

Antigen Details

Structure
Integrin α chain, 150 kD
Distribution

NK cells, subset of T cells

Function
Adhesion
Ligand/Receptor
Collagen, laminin
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
3. Sasaki K, et al. 2003. Int. Immunol. 15:701.
4. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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