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Purified anti-mouse H-2Ld/H-2Db Antibody

Pricing & Availability
Clone
28-14-8 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H/HeJ) IgG2a, κ
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Product Citations
publications
28-14-8
C57BL/6 mouse splenocytes stained with 28-14-8 PE
  • 28-14-8
    C57BL/6 mouse splenocytes stained with 28-14-8 PE
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114502 500 µg 247 CHF
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Description

The 28-14-8 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2Ld haplotype. Clone 28-14-8 recognizes the α3 domain of H-2Db.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114510).

Does not react with other haplotypes (e.g., k, f, p, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1980. J. Immunol. 125:2473.
  2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
  3. Marguet D, et al. 1999. Immunity 11:231.
  4. Bashuda H, et al. 1997. Transplantation 63:113.
  5. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed
  6. Milstein O, et al. 2011. Blood 117:1042.
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Azuma T, et al. 2008. Blood. 111:3635. PubMed
  3. Frey BF, et al. 2018. J Immunol. 200:1853. PubMed
  4. Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
RRID
AB_313583 (BioLegend Cat. No. 114502)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Ld/H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Ld/H-2Db on UniProt.org
Go To Top Version: 2    Revision Date: 08.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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