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Purified anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
W18174A (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18174A_PURE_Ly-6AE_Antibody_081220
C57BL/6 mouse splenocytes were stained with purified anti-mouse Ly-6A/E (clone W18174A) (filled histogram) or purified anti-rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE antibody.
  • W18174A_PURE_Ly-6AE_Antibody_081220
    C57BL/6 mouse splenocytes were stained with purified anti-mouse Ly-6A/E (clone W18174A) (filled histogram) or purified anti-rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE antibody.
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160902 100 µg 101 CHF
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
EL4 Cell
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes.

Product Citations
  1. Hammers DW, et al. 2022. JCI Insight. 7:. PubMed
RRID
AB_2876574 (BioLegend Cat. No. 160902)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells, Thymocytes
Biology Area
Stem Cells
Antigen References
  1. Upadhyay G. 2019. Front Immunol. 10:819.
  2. Rock KL, et al. 1989. Immunol. Rev. 111:195-224.
Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 1    Revision Date: 08.12.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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