Purified anti-mouse Ly49D Antibody anti-Ly49D

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Clone: 4E5 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-49D
Isotype: Rat IgG2a, κ
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Product Citations: publications

4E5_FITC_1_051710 — C57BL/6 splenocytes stained with CD49b (DX5) APC and purified 4E5 conjugated with FITC

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138302

500 µg

293 CHF

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Description

Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily. Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J. The receptors form homodimers that bind to MHC class I alloantigens. Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif. The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-D^d, D^r and D^sp2, but not with H2^b or H2^kclass I antigens. Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells. In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation¹ and blocking^{2, 3}of CHO cell or Con A-activated lymphoblast killing by NK cells.
RRID: AB_10574460 (BioLegend Cat. No. 138302)

Antigen Details

Structure: A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution: Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
Function: Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand/Receptor: MHC class I alloantigens
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.
Gene ID: 16635 View all products for this Gene ID
UniProt: View information about Ly49D on UniProt.org

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Other Formats

View All Ly49D Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ly49D	4E5	FC,IP
FITC anti-mouse Ly49D	4E5	FC
Alexa Fluor® 647 anti-mouse Ly49D	4E5	FC
PE anti-mouse Ly49D	4E5	FC
TotalSeq™-A0841 anti-mouse Ly49D	4E5	PG
TotalSeq™-C0841 anti-mouse Ly49D	4E5	PG
TotalSeq™-B0841 anti-mouse Ly49D	4E5	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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