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Purified anti-Nos2 (iNOS) Antibody

Pricing & Availability
Clone
W16030C (See other available formats)
Regulatory Status
RUO
Other Names
Nitric oxide synthase 2, nitric oxide synthase 2A, NOS2A, HEP-NOS, inducible nitric oxide synthase (iNOS), Nitric oxide synthase, inducible, Inducible NOS
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16030C_PURE_Nos2_Antibody_1_WB_042017
Total cell lysates (15 µg protein) from non-treated and LPS treated (1 µg/mL for 16 hours) Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.02 µg/mL of purified anti-Nos2 (clone W16030C) antibody and a competitor's antibody used at the manufacture's recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16030C and a donkey anti-rabbit IgG antibody conjugated to HRP for the competitor’s antibody. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower). Lane M is the molecular ladder.
  • W16030C_PURE_Nos2_Antibody_1_WB_042017
    Total cell lysates (15 µg protein) from non-treated and LPS treated (1 µg/mL for 16 hours) Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.02 µg/mL of purified anti-Nos2 (clone W16030C) antibody and a competitor's antibody used at the manufacture's recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16030C and a donkey anti-rabbit IgG antibody conjugated to HRP for the competitor’s antibody. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower). Lane M is the molecular ladder.
  • W16030C_PURE_Nos2_Antibody_2_IF_042017
    Non-treated (left image) and LPS treated (1 µg/mL for 16 hours, right image) Raw264.7 cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.25 µg/mL anti-Nos2 antibody (clone W16030C) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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696802 100 µg 276 CHF
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Description

Nitric Oxide Synthase 2 (NOS2), also known as inducible NOS (iNOS), contains an N-terminal oxygenase domain and a C-terminal reductase domain, and functions to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a reactive free radical which acts as a biologic messenger with diverse functions throughout the body, such as neurotransmission, antimicrobial, and antitumor activity. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. NOS2 also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such as COX2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse NOS2 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.002 - 0.02 µg per ml. For immunocytochemistry, a concentration range of 0.05 - 0.5 µg per ml is suggested. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Mouse NOS2 has two isoforms: Isoform A is 130 kD and isoform B is 118 kD. This clone recognizes isoform B better than A in the tested cell.

Product Citations
  1. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  2. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
  3. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  4. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
RRID
AB_2687097 (BioLegend Cat. No. 696802)

Antigen Details

Structure
1144 amino acids with a predicted molecular weight of 130 kD. Contains a flavodoxin-like domain and a FAD-binding FR-type domain.
Distribution

Cytoplasm

Function
NOS2 is a nitric oxide synthase and produces large quantity of NO in response to the stimulation of proinflammatory cytokines. NOS2 plays an important role in host defense against pathogens and participates in anti-tumor processes.
Interaction
Forms a homodimer. Interacts with SLC9A3R1, GAPDH, S100A8, and S100A9.
Biology Area
Cell Biology, Immunology, Innate Immunity
Antigen References

1. Salim T, et al. 2016. PLoS One. 11:e0153289.
2. Ma Z, et al. 2016. Inflammation. 39:543.
3. Lee SH, et al. 2016. J. Cell. Biochem. 117:978.
4. Landes MB, et al. 2015. J. Leuko. Biol. 97:1111.
5. Karpuzoglu E, et al. 2006. Nitric. Oxide 15:177.
6. Suschek CV, et al. 2004. Curr. Mol. Med. 4:763.

Gene ID
4843 View all products for this Gene ID
UniProt
View information about Nos2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07.27.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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