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Purified anti-PKCι Antibody

Pricing & Availability
Clone
W18205A (See other available formats)
Regulatory Status
RUO
Other Names
Protein Kinase C iota, Atypical Protein Kinase C-lambda/iota, Protein Kinase C iota Type, PRKC-lambda/iota, aPKC-lambda/iota, atypical protein kinase C, aPKC, aPKCλ/ι
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18205A_PURE_PKCi_Antibody_1_103019
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PKCι antibody (clone W18205A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of ascending PRKCI expression; predicted expression data obtained from Human Protein Atlas.
  • W18205A_PURE_PKCi_Antibody_1_103019
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PKCι antibody (clone W18205A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of ascending PRKCI expression; predicted expression data obtained from Human Protein Atlas.
  • W18205A_PURE_PKCi_Antibody_2_103019
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400602) or purified anti-PKCι antibody (clone W18205A). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of clone W18205A. The secondary step was performed using light-chain-specific secondary antibodies. Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
671201 25 µg 101 CHF
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671202 100 µg 253 CHF
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Description

Members of the protein kinase c family (PKC) are serine/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PKCι protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.

RRID
AB_2820114 (BioLegend Cat. No. 671201)
AB_2820114 (BioLegend Cat. No. 671202)

Antigen Details

Structure
PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.
Distribution

Cytosol and Nucleus/Ubiquitously expressed

Function
Protein Kinase/Cell Signaling
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Nayak RC, et al. 2019. Nat. Commun. 10:46.
  2. Riddell M, et al. 2018. Nat. Commun. 9:5357.
  3. Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872.
  4. Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190.
  5. Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.
Gene ID
5584 View all products for this Gene ID
UniProt
View information about PKCiota on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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