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Purified anti-S100A4 Antibody (Previously Covance catalog# PRB-497P)

Pricing & Availability
Clone
S100A4 (See other available formats)
Regulatory Status
RUO
Other Names
Protein S100-A4, calvasculin, protein 18A2, protein Mts1, metastatic cell protein, S100 calcium-binding protein A4, placental calcium-binding protein, leukemia multidrug resistance associated protein, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Previously
Covance Catalog# PRB-497P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
S100A4_Purified_S100A4_IHC_020415.jpg
Staining of PRB-497P on formalin-fixed paraffin embedded human tonsil.
  • S100A4_Purified_S100A4_IHC_020415.jpg
    Staining of PRB-497P on formalin-fixed paraffin embedded human tonsil.
Cat # Size Price Quantity Check Availability Save
810101 250 µL 386 CHF
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Description

The S100A4 protein belongs to the S100 subfamily, which has grown to be one of the largest subfamilies of the EF-hand Ca2+-binding proteins. The S100 proteins are small (10-12 kD), acidic proteins sharing 25-65% sequence homology. A majority of the S100 genes cluster on human chromosome 1q21. The discovery of this cluster has provided the basis for a new nomenclature for S100 proteins encoded by genes in the 1q21 region. These proteins are now designated S100A1-S100A13. In immunoblotting analysis using recombinant S100 proteins (S100A1, S100A2, S100A4, S100A6 and S100B) only the corresponding antigen (S100A4) was recognized. No cross-reaction with the other S100 proteins was observed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Cat, Cow, Dog
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against purified recombinant human S100A4 monomer expressed by E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.1 M NaCl, 0.015 M sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2 - 8°C
Application

IHC-P, ICC
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition. 
• IHC-P: 1:100 - 1:200 for Biotin based detection systems such as USA Ultra Streptavidin Detection (929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval Solution (928502).
Incubation: 20 minutes at room temperature. 

Application Notes

This antibody is effective in immunocytochemistry (ICC) and immunohistochemistry (IHC).

Predicted Molecular Weight = 10 - 12 kD

Postive Tissue: Tonsil (human)  


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-40, 1996.
  2. Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CV. Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci 90:6547-51, 1993.
Product Citations
  1. Seldin L, et al. 2020. Developmental Cell. 55(5):558-573.e6. PubMed
  2. Lee DF, et al. 2018. Sci Rep. 8:11927. PubMed
  3. Chen L, et al. 2020. Cancer Immunol Res. 1381:8. PubMed
RRID
AB_2564748 (BioLegend Cat. No. 810101)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Immune Checkpoint Receptors
Gene ID
20198 View all products for this Gene ID
UniProt
View information about S100A4 on UniProt.org
Go To Top Version: 5    Revision Date: 08.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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