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Purified anti-SOD1 Antibody

Pricing & Availability
Clone
O98B10 (See other available formats)
Regulatory Status
RUO
Other Names
Superoxide dismutase 1, Superoxide dismutase [Cu-Zn] , Superoxide Dismutase, Cystolic
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O98B10_PURE_SOD1_Antibody_WB_083117
Western blots of anti-SOD1 antibody (clone O98B10) and isotype-matched control mouse IgG2b. Lanes 1 and 2: 20 µg of normal human brain lysates; Lanes 3 and 4: 20 µg of mouse brain lysates. Blots were incubated overnight at 4°C with 1 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • O98B10_PURE_SOD1_Antibody_WB_083117
    Western blots of anti-SOD1 antibody (clone O98B10) and isotype-matched control mouse IgG2b. Lanes 1 and 2: 20 µg of normal human brain lysates; Lanes 3 and 4: 20 µg of mouse brain lysates. Blots were incubated overnight at 4°C with 1 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • O98B10_PURE_SOD1_Antibody_WB_2_051519
    Western blot of purified anti-SOD1 antibody (clone O98B10). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
850701 25 µg 106 CHF
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850702 100 µg 265 CHF
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Description

Superoxide dismutase 1 (SOD1) is one of three isozymes responsible for destroying free superoxide radicals in the body. SOD1 is a homodimeric cytoplasmic Cu/Zn binding protein that converts harmful superoxide radicals to oxygen and hydrogen peroxide. The other isozymes are SOD2 and SOD3, which are localized to the mitochondria and extracellular space, respectively. SOD1 mutations are associated with familial amyotrophic lateral sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human SOD1 expressed in 293T cells transfected with human SOD1 expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 1 - 5 µg/ml in human, mouse, and rat and 0.5 - 10 µg/ml in Drosophila.

RRID
AB_2716015 (BioLegend Cat. No. 850701)
AB_2716015 (BioLegend Cat. No. 850702)

Antigen Details

Structure
SOD1 is a homodimeric β-barrel protein that contains an intramolecular disulfide bond and a binuclear Cu/Zn site in each subunit. SOD1 monomers have an apparent molecular weight of ~16 kD.
Distribution

Tissue sources: Ubiquitously expressed.  
Cellular distribution: Cytosol, mitochondrion, peroxisome and nucleus.

Function
SOD1 converts harmful superoxide radicals in the body to oxygen and hydrogen peroxide.
Interaction
UBC, CCS, PRDX1, HSPA4
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Enzymes and Regulators
Antigen References

1. Lee S, Kim HJ. 2015. Exp. Neurobiol. 24(1):1-7.
2. Tafuri F, et al. 2015. Front Cell Neurosci. 9: 336.
3. Palomo, GM, Manfredi G. 2015. Brain Res. 1607:36-46.
4. Rosen DR, et al. 1993. Nature 362 (6415):59-62.

Gene ID
6647 View all products for this Gene ID
UniProt
View information about SOD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05.15.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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