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Purified anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C2C34 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7C2C34_PURE_SPI1_PU.1_Antibody_1_WB_070918
Total cell lysates (15 µg protein) from THP1 (lane 1) and Raw264.7 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-SPI1 (PU.1) Antibody, clone 7C2C34 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated with HRP for anti-SPI1 (PU.1) Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower) as a loading control. Lane M: Molecular weight ladder. M* Molecular weight ladder with longer exposure.
  • 7C2C34_PURE_SPI1_PU.1_Antibody_1_WB_070918
    Total cell lysates (15 µg protein) from THP1 (lane 1) and Raw264.7 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-SPI1 (PU.1) Antibody, clone 7C2C34 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated with HRP for anti-SPI1 (PU.1) Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower) as a loading control. Lane M: Molecular weight ladder. M* Molecular weight ladder with longer exposure.
  • 7C2C34_PURE_SPI1_PU.1_Antibody_2_IF_100115
    Raw 264.7 cells were stained with purified anti-SPI1 (clone 7C2C34) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-rat IgG (red) antibody and Alexa Fluor® 488 anti-Tubulin-α antibody (green).
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681301 25 µg 101 CHF
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681302 100 µg 241 CHF
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Description

SPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length SPI1 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566468 (BioLegend Cat. No. 681301)
AB_2566468 (BioLegend Cat. No. 681302)

Antigen Details

Structure
272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757.

Gene ID
20375 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org
Go To Top Version: 1    Revision Date: 10.01.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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