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Purified anti-Tau, 368-441 Antibody

Pricing & Availability
Clone
A16097F (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule associated protein tau
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
A16097F_Purified_Tau368-441_Antibody_1_110917
Western blots of anti-Tau, 368-441 antibody (clone A16097F) and rat IgG2a isotype control. Lane 1: 20 µg of normal human brain lysate; Lane 2: 50 ng of recombinant human Tau ladder. The blots were incubated overnight at 4°C with 1 µg/ml of anti-Tau or rat IgG2a, followed by incubation with horseradish peroxidase labeled goat anti-rat IgG secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A16097F_Purified_Tau368-441_Antibody_1_110917
    Western blots of anti-Tau, 368-441 antibody (clone A16097F) and rat IgG2a isotype control. Lane 1: 20 µg of normal human brain lysate; Lane 2: 50 ng of recombinant human Tau ladder. The blots were incubated overnight at 4°C with 1 µg/ml of anti-Tau or rat IgG2a, followed by incubation with horseradish peroxidase labeled goat anti-rat IgG secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A16097F_Purified_Tau368-441_Antibody_2_110917
    IHC staining of anti-Tau, 368-441 antibody (clone A16097F) and rat IgG2a isotype control on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with 5.0 µg/ml of anti-Tau or rat IgG2a overnight at 4°C. Biotinylated anti-rat IgG, HRP Streptavidin, and DAB (3,3'-diaminobenzidine) substrate were used as the detection system. Slides were counterstained with hematoxylin.
  • A16097F_Purified_Tau368-441_Antibody_3_110917
    Direct ELISA of purified anti-Tau, 368-441 antibody (clone A16097F) and rat IgG2a isotype control binding to plate-immobilized recombinant human 2N3R and 2N4R Tau proteins. ELISA was performed by coating wells with 100 ng of each recombinant Tau protein. The wells were then incubated with anti-Tau or ratIgG2a at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
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851201 25 µg 112 CHF
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851202 100 µg 276 CHF
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 231-441.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. For Direct ELISA, a concentration range of 0.01 - 1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with human 3R and 4R Tau isoforms, and recognizes endogenous human Tau in brain lysates by WB. The antibody has minimal reactivity with mouse Tau protein.

RRID
AB_2721762 (BioLegend Cat. No. 851201)
AB_2721762 (BioLegend Cat. No. 851202)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
  3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau 368-441 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 11.17.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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