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Purified anti-Tau Phospho (Thr181) Antibody

Pricing & Availability
Clone
M7004D06 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule associated protein tau
Isotype
Mouse IgG1, κ
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Product Citations
publications
M7004D06_PURE_TauPhospho_Antibody_1_041618
IHC staining of anti-Tau Phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue, and lack of staining of normal brain tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 10 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection.
  • M7004D06_PURE_TauPhospho_Antibody_1_041618
    IHC staining of anti-Tau Phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue, and lack of staining of normal brain tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 10 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection.
  • M7004D06_PURE_TauPhospho_Antibody_2_041618
    IHC staining of purified anti-Tau phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody for one hour at room temperature, followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with Hoechst stain. The image was captured by 40X objective. Scale bar: 50 µm
  • M7004D06_PURE_TauPhospho_Antibody_3_041618
    Western blot of anti-Tau Phospho (Thr181) (clone M7004D06), anti-Tau Phospho (Ser396) (clone PHF-13) antibodies, and isotype-matched control IgG1 and IgG2b. Lane 1: 20 µg of Parkinson’s disease human brain lysate; Lane 2: 2 µg of Tau paired helical filaments. Blots were incubated overnight at 4°C with 10 µg/ml of the primary antibody, followed by horseradish peroxidase labeled goat anti-mouse secondary antibody. Western blot of anti-Tau Phospho (Ser396) (clone PHF-13) serves as a positive control for Tau staining.
  • M7004D06_PURE_TauPhospho_Antibody_4_052318_updated
    Direct ELISA of purified anti-Tau Phospho (Thr181) antibody (clone M7004D06) binding to plate-immobilized human phospho-tau (Thr181) peptide and scrambled sequence control peptide. ELISA was performed by coating wells with 150 ng of phospho-tau or scrambled peptides. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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846601 25 µg 112 CHF
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846602 100 µg 300 CHF
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Recent studies indicate that cerebrospinal fluid tau phosphorylated at position threonine 181 has diagnostic utility for several neurological disorders. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
15-mer pT181 Tau peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. For Western blotting, a concentration range of 2.0 - 10 μg/ml is suggested. For Direct ELISA, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed
RRID
AB_2616881 (BioLegend Cat. No. 846601)
AB_2616881 (BioLegend Cat. No. 846602)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Molecular Family
Phospho-Proteins, Tau
Antigen References

1. Meredith JE Jr, et al. 2013. PLoS One 8(10):e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1):89. PubMed

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho Thr181 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 03.21.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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