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- Regulatory Status
- RUO
- Other Names
- Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, MER6, Integrin-associated protein, IAP, CD47 glycoprotein, CD47 antigen
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- Product Citations
- publications
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When mouse SIRPα is coated at 2 µg/mL (100 µL/well), mouse CD47-Fc Chimera binds with an EC50 of 25 - 100 ng/mL. -
Stability testing for mouse CD47-Fc Chimera. Recombinant mouse CD47-Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (control). The samples were tested in a binding assay with mouse SIRPα.
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CD47 (Cluster of differentiation 47), also known as integrin-associated protein (IAP), is a ~50 kD glycosylated protein belonging to the immunoglobulin superfamily. It is a ubiquitously expressed integral membrane protein with a single IgV-like domain at its N-terminus, five membrane-spanning regions, and an alternatively spliced cytoplasmic C-terminus. CD47 was first identified as a protein involved in β3 integrin-mediated signaling in leukocytes, but its known to interact with SIRPα (signal regulatory protein-alpha), TSP-1 (thrombospondin-1), and other integrin members to regulate cell migration, axon extension, cytokine production, and T cell activation. CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. These interactions function as a negative immune checkpoint to prevent the macrophage-mediated clearance of healthy CD47-expressing cells. CD47 also associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis. CD47 and Fas ligation promotes T cell anergy and dampens Th1 immune responses.
Product DetailsProduct Details
- Source
- Mouse CD47, amino acid Gln19-Lys140 (Accession # Q61735), with a C-terminal hIgG1 Fc tag, was expressed in CHO cells.
- Molecular Mass
- The 361 amino acid recombinant protein has a predicted molecular mass of approximately 40.7 kD. The DTT-reduced protein migrates at approximately 55 kD and and non-reduced protein migrates at approximately 110 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When mouse SIRPα is coated at 2 µg/mL (100 µL/well), mouse CD47-Fc Chimera binds with an EC50 of 25 – 100 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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Very broadly distributed in normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
- Function
- Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins
- Interaction
- Macropahges, dendritic cells, platelets
- Ligand/Receptor
- SIRPα, THBS1, fibrinogen
- Bioactivity
- Measured by its ability to bind SIPRα
- Cell Type
- Endothelial cells, Epithelial cells, Fibroblasts, Neurons, Platelets
- Biology Area
- Cell Adhesion, Immuno-Oncology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
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- Wu AL, et al. 1999. Mol. Cell. 4:619-25.
- Han X, et al. 2000. J. Biol. Chem. 275:37984-92.
- Latour S, et al. 2001. J. Immunol. 167:2547-54.
- Piccio L, et al. 2005. Blood 6:2421-7.
- Hatherley D, et al. 2008. Mol. Cell 31:266-77.
- Casey SC, et al. 2016. Science 352:227-31.
- Kojima Y, et al. 2016. Nature 536:86-90.
- Gene ID
- 16423 View all products for this Gene ID
- UniProt
- View information about CD47 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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