Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
A_Poly4053_SparkRed718_IgG_Antibody_IHCP_101923
IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Poly4053) (magenta) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated 5 μg/mL of purified anti-GFAP (Cat. No. 644702) followed by incubation with 10 μg/mL of Spark Red™ 718 Goat anti-mouse IgG for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). Images were captured with a 10X (panel A) and 40X (panel B) objective. Scale bar: 50 μm
  • A_Poly4053_SparkRed718_IgG_Antibody_IHCP_101923
    IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Poly4053) (magenta) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated 5 μg/mL of purified anti-GFAP (Cat. No. 644702) followed by incubation with 10 μg/mL of Spark Red™ 718 Goat anti-mouse IgG for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). Images were captured with a 10X (panel A) and 40X (panel B) objective. Scale bar: 50 μm
  • B_Poly4053_SparkRed718_IgG_Antibody_IHCF_101923
    IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Poly4053) (magenta) on frozen rat brain. Following 4% PFA fixation, the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of purified anti-GFAP (Cat. No. 644702) followed by incubation with 10 μg/mL of Spark Red™ 718 Goat anti-mouse IgG for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • C_Poly4053_SparkRed718_IgG_Antibody_ICC_101923
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5 μg/mL of purified anti-Cofilin 1 (Cat. No. 865302), followed by incubation with 10 μg/mL Spark Red™ 718 Goat anti-mouse IgG (clone Poly4053) (orange, panel A). Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) for 1 hour at room temperature and nuclei were counterstained with DAPI (Cat. No. 422801) (merge in panel B). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 μm
Compare all formats See Spark Red™ 718 spectral data
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405318 200 µg 350 CHF
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
IHC-F, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
RRID
AB_3097256 (BioLegend Cat. No. 405318)

Other Formats

View All IgG Reagents Request Custom Conjugation
Description Clone Applications
APC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Biotin Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
FITC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
HRP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,IHC,WB
PE Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Purified Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,FC,WB,IHC
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC,IHC-F,IHC-P
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-F
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 IHC-P,IHC-F,ICC
Go To Top Version: 1    Revision Date: 10.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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