- Clone
- C8/144B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Leu2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C8slash144B_SPKYG570_CD8a_Antibody_120321 C8slash144B_SPKYG570_CD8a_Antibody_120321](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C8slash144B_SPKYG570_CD8a_Antibody_120321.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD8a (clone C8/144B) Spark YG™ 570 (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
372909 | 25 µg | 106 CHF | ||||
372910 | 100 µg | 265 CHF |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include activation3, flow cytometry4, immunohistochemical staining of frozen tissue sections2, and Western blotting5.
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Application References
(PubMed link indicates BioLegend citation) -
- McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88. (IHC-P)
- Petukhova L, et al. 2010. Nature 466:113. (IHC-F)
- Clement M, et al. 2011. J. Immunol. 187:654. (Activ)
- MarTchal R, et al. 2010. BMC Cancer 10:340. (FC)
- Pontiggia L, et al. 2008. J. Invest. Dermatol. 129:480. (WB)
- RRID
-
AB_2904405 (BioLegend Cat. No. 372909)
AB_2904405 (BioLegend Cat. No. 372910)
Antigen Details
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β.
- Distribution
-
Majority of thymocytes, T cell subset, NK cells.
- Function
- MHC class I co-receptor, thymic differentiation, T-cell activation.
- Ligand/Receptor
- MHC class I molecules.
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88.
- Gene ID
- 100387674 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
Related FAQs
Other Formats
View All CD8a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD8a | C8/144B | IHC-P,Activ,FC,IHC-F,WB |
Alexa Fluor® 594 anti-human CD8a | C8/144B | IHC-P,SB |
Alexa Fluor® 647 anti-human CD8a | C8/144B | IHC-P,ICFC,3D IHC,SB |
Biotin anti-human CD8a | C8/144B | IHC-P |
Spark YG™ 570 anti-human CD8a | C8/144B | IHC-P |
TotalSeq™-Bn1320 anti-human CD8a | C8/144B | SB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD8a
Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD8a
Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 647 anti-human CD8a
Human paraffin-embedded tonsil tissue slices were prepared w... Human peripheral blood mononuclear cells were surface staine... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
Biotin anti-human CD8a
Human paraffin-embedded tonsil tissue slices were prepared w... -
Spark YG™ 570 anti-human CD8a
Human paraffin-embedded spleen tissue slices were prepared w... -
TotalSeq™-Bn1320 anti-human CD8a
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