TotalSeq™-D0147 anti-human CD62L antibody, CD62L, DREG-56

Pricing & Availability

Clone: DREG-56 (See other available formats)
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ
Barcode Sequence: GTCCCTGCAACTTGA
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Product Citations: publications

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304863

10 µg

369 CHF

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Description

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting^2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)². The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2894612 (BioLegend Cat. No. 304863)

Antigen Details

Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.

Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402 View all products for this Gene ID
UniProt: View information about CD62L on UniProt.org

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All CD62L Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD62L	DREG-56	FC
FITC anti-human CD62L	DREG-56	FC
PE anti-human CD62L	DREG-56	FC
PE/Cyanine5 anti-human CD62L	DREG-56	FC
Purified anti-human CD62L	DREG-56	FC,WB,Block
APC/Cyanine7 anti-human CD62L	DREG-56	FC
Alexa Fluor® 488 anti-human CD62L	DREG-56	FC
Alexa Fluor® 647 anti-human CD62L	DREG-56	FC
Alexa Fluor® 700 anti-human CD62L	DREG-56	FC
PE/Cyanine7 anti-human CD62L	DREG-56	FC
PerCP/Cyanine5.5 anti-human CD62L	DREG-56	FC
Pacific Blue™ anti-human CD62L	DREG-56	FC
Brilliant Violet 421™ anti-human CD62L	DREG-56	FC
Brilliant Violet 785™ anti-human CD62L	DREG-56	FC
Brilliant Violet 650™ anti-human CD62L	DREG-56	FC
PE/Dazzle™ 594 anti-human CD62L	DREG-56	FC
Brilliant Violet 605™ anti-human CD62L	DREG-56	FC
Purified anti-human CD62L (Maxpar® Ready)	DREG-56	FC,CyTOF®
APC/Fire™ 750 anti-human CD62L	DREG-56	FC
Brilliant Violet 510™ anti-human CD62L	DREG-56	FC
TotalSeq™-A0147 anti-human CD62L	DREG-56	PG
TotalSeq™-B0147 anti-human CD62L	DREG-56	PG
TotalSeq™-C0147 anti-human CD62L	DREG-56	PG
Ultra-LEAF™ Purified anti-human CD62L	DREG-56	FC,Block,WB
Brilliant Violet 711™ anti-human CD62L	DREG-56	FC
Spark NIR™ 685 anti-human CD62L	DREG-56	FC
TotalSeq™-D0147 anti-human CD62L	DREG-56	PG
APC/Fire™ 810 anti-human CD62L	DREG-56	FC
FITC anti-human CD62L	DREG-56	FC
PE/Dazzle™ 594 anti-human CD62L	DREG-56	FC
PerCP/Fire™ 806 anti-human CD62L	DREG-56	FC
APC anti-human CD62L	DREG-56	FC
GMP FITC anti-human CD62L	DREG-56	FC
Biotin anti-human CD62L	DREG-56	FC
Spark Blue™ 574 anti-human CD62L (Flexi-Fluor™)	DREG-56	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.