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Veri-Cells™ PBMC (CD103, CD117)

Pricing & Availability
Regulatory Status
RUO
Other Names
Integrin αE, integrin αE (CD103)β7, control cells
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Product Citations
publications
A. VeriCells_PBMC_CD103-CD117_1_052224
Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5 and anti-human CD19 (clone HIB19) FITC (right). Cells were gated on forward and side scatter (left).
  • A. VeriCells_PBMC_CD103-CD117_1_052224
    Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5 and anti-human CD19 (clone HIB19) FITC (right). Cells were gated on forward and side scatter (left).
  • B. VeriCells_PBMC_CD103-CD117_3_052224
    Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD1a (clone HI149) Brilliant Violet 711™ (left) or mouse IgG1, κ Brilliant Violet 711™ isotype control (right). CD14- CD19- double negative single cells are shown.
  • C. VeriCells_PBMC_CD103-CD117_4_052224
    Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD117 (clone 104D2) PE (left) or mouse IgG1, κ PE isotype control (right). CD14- CD19- double negative single cells are shown.
  • D. VeriCells_PBMC_CD103-CD117_2_052224
    Veri-Cells™ CD103 PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD103 (clone Ber-ACT8) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). CD14- CD19- double negative single cells are shown.
  • E. VeriCells_PBMC_CD103-CD117_5_052224
    Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD30 (clone BY88) APC (left) or mouse IgG1, κ APC isotype control (right). CD14- CD19- double negative single cells are shown.
  • F. VeriCells_PBMC_CD103-CD117_6_052224
    Veri-Cells™ PBMC (CD103, CD117) were stained with anti-human CD14 (clone M5E2) PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD71 (clone CY1G4) Brilliant Violet 650™ (left) or mouse IgG2a, κ Brilliant Violet 650™ (right). CD14- CD19- double negative single cells are shown.
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425005 10 tests 140 CHF
 
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Description

Reconstituted Veri-Cells™ PBMC (CD103, CD117) can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for stability studies to avoid donor variability. Veri-Cells™ PBMC (CD103, CD117) has been verified to express commonly tested cell surface (CD3, CD4, CD8, CD19, CD56) markers as well as “rare antigens” CD103, CD117, CD1a, CD30 and CD71.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA-approved methods for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus, HTLV (Human T-Lymphotropic virus I and II), surface antigen for Hepatitis B (HBsAg) virus, syphilis, and the West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting diseases. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Formulation
2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC) and 1 vial of 0.7 mL Veri-Cells™ Buffer A Plus.
Preparation
The Veri-Cells™ (CD103, CD117) PBMC kit consists of 2 lyophilized human Veri-Cells™ (CD103, CD117) PBMC vials and a vial of Veri-Cells™ Buffer A Plus.
Concentration
≥ 1 X 106 cells per vial (lyophilized)
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Application

FC - Quality tested
Recommended Usage

50 µL per test (after reconstitution with 325 µL of Veri-Cells™ Buffer A Plus).

Note: Veri-Cells™ Buffer A Plus may show precipitation over time. However, this is normal and does not affect the performance of the buffer.

Application Notes

Veri-Cells™ PBMC (CD103, CD117) can be used as a staining control that provides assay values for CD103, CD1a, CD117, CD30 and CD71 expression. For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for all washing steps. Please note that any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.
 

Cell Subset Acceptance Criteria
CD103+ 10%-60%
CD1a+ 1%-60%
CD117+ 0.1%-5%
CD30+ 0.3%-20%
CD71+ 60%-90%



Note on indicated ranges: Percent positive values are based on gates set on isotype controls. It is recommended that individual laboratories establish their own control values and ranges for each parameter based on laboratory-specific conditions. 

The antibodies used for testing are the following:

Cat. No. Marker clone Format
301863 / 301864 CD14 M5E2 PE/Cyanine5
302205 / 302206 CD19 HIB19 FITC
350211 / 350212 CD103 Ber-ACT8 PE/Cyanine7
300139 / 300140 CD1a HI149 Brilliant Violet 711™
333909 / 333910 CD30 BY88 APC
334115 / 334116 CD71 CY1G4 Brilliant Violet 650™
313203 / 313204 CD117 104D2 PE



Please use our CoA Look-Up Tool to find the expected frequencies of each markers for each lot as determined by analysis of the positive single cells. The values reported on the Certificate of Analysis are provided as a reference only. Variability in antibodies used, instruments, staining conditions, and other factors may influence the end user values.

Antigen Details

Gene ID
NA

Related FAQs

Is washing required after staining?

Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.

Can the cells be activated after rehydration?
No, they cannot be activated.
Are these cells virus/pathogen free?
These are tested to be free of HIV, HBV, syphilis, and HCV.
Are there any RBCs in Veri-cells™?
There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
Are these cells viable?
No they are not.
Can a constant percentage of each population be expected in each lot?
Yes, within each lot, cell populations remain constant.
Are the cells fixed?

They are treated with our proprietary mixture prior to lyophilization.

Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?

For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.

How do we QC these cells?

Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range.  For more information regarding the exact markers used for each product, please contact technical service.

Go To Top Version: 1    Revision Date: 05.21.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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