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APC anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1_APC_CD137_Antibody_051018
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with CD137 (clone 4B4-1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 4B4-1_APC_CD137_Antibody_051018
    PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with CD137 (clone 4B4-1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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309809 25 tests 144 CHF
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309810 100 tests 332 CHF
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Tarke A, et al. 2021. Cell Reports Medicine. 2(2):100204. PubMed
  2. Apostolidis SA, et al. 2021. Nat Med. 27:1990. PubMed
  3. Nielsen MA, et al. 2022. Front Immunol. 13:915890. PubMed
  4. Hong CH, et al. 2022. Front Immunol. 13:1021067. PubMed
  5. Yu ED, et al. 2022. Cell Rep Methods. 2:100350. PubMed
  6. Eschweiler S 2023. Cell Reports. 42(2):112040. PubMed
  7. Terahara K, et al. 2022. iScience. 25:104959. PubMed
  8. Sun P, et al. 2022. iScience. 25:105202. PubMed
  9. Fendler A, et al. 2022. Cell Rep Med. 3:100781. PubMed
  10. Ryan FJ, et al. 2023. Cell Rep Med. 4:100971. PubMed
  11. Nguyen THO, et al. 2023. Cell Rep Med. 4:101017. PubMed
  12. Xiao C, et al. 2023. Nat Aging. 3:418. PubMed
  13. Zhang W, et al. 2023. Nat Immunol. 24:966. PubMed
  14. Tarke A, et al. 2023. Cell Rep Med. 4:101088. PubMed
  15. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  16. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  17. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  18. Grifoni A, et al. 2020. J Virol. 94:00:00. PubMed
  19. van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
  20. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  21. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  22. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  23. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
  24. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  25. Painter MM, et al. 2021. Immunity. 54:2133. PubMed
  26. Zhang J, et al. 2022. Nature. 609:369. PubMed
  27. Tarke A, et al. 2022. Cell. 185:847. PubMed
  28. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  29. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  30. Valenzuela NM, et al. 2021. Front Immunol. 12:648946. PubMed
  31. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  32. Voic H, et al. 2020. J Virol. 94: . PubMed
  33. Kishton RJ, et al. 2022. Cell Rep. 40:111153. PubMed
  34. Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed
  35. Martin SD, et al. 2018. Oncoimmunology. 7:e1371895. PubMed
  36. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  37. Mo F, et al. 2021. Nat Biotechnol. 56:39. PubMed
  38. Tarke A, et al. 2022. Int J Mol Sci. 23:. PubMed
  39. Lederer K, et al. 2022. Cell. . PubMed
  40. Schouest B, et al. 2021. J Virol. 95:. PubMed
  41. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  42. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  43. Eriksen LL, et al. 2021. PLoS One. 16:e0255574. PubMed
  44. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  45. Nuffel A, et al. 2012. J Immunol Methods. 377:23. PubMed
  46. Yu ED, et al. 2022. Cell Host Microbe. . PubMed
  47. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_830671 (BioLegend Cat. No. 309809)
AB_830671 (BioLegend Cat. No. 309810)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 2    Revision Date: 05.10.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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