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Brilliant Violet 421™ anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4S.B3_BV421_1_040311
Human peripheral blood lymphocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), surface stained with CD3 FITC, fixed, permeabilized, and then stained with IFN-γ (clone 4S.B3) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4S.B3_BV421_1_040311
    Human peripheral blood lymphocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), surface stained with CD3 FITC, fixed, permeabilized, and then stained with IFN-γ (clone 4S.B3) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4S.B3_BV421_2_040311
Compare all formats See Brilliant Violet 421™ spectral data
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502531 25 tests 198 CHF
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502532 100 tests 402 CHF
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Pal R, et al. 2020. Curr Protoc Cytom. 92:e69. PubMed
  2. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  3. Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
  4. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  5. Wu B, et al. 2020. Cell Metabolism. 32(6):967-980.e5. PubMed
  6. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  7. Chalan P, et al. 2016. J Rheumatol. 43: 1008 - 1016. PubMed
  8. Felices M, et al. 2018. JCI Insight. 3. PubMed
  9. Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed
  10. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  11. Langenberg MCC, et al. 2020. Nat Med. 26:326. PubMed
  12. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  13. Zelba H, et al. 2021. J Immunol. 206:580. PubMed
RRID
AB_10900083 (BioLegend Cat. No. 502531)
AB_10900083 (BioLegend Cat. No. 502532)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human IFN-γ 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
FITC anti-human IFN-γ 4S.B3 ICFC
Biotin anti-human IFN-γ 4S.B3 ELISA Detection,ELISPOT Detection,ICFC
Purified anti-human IFN-γ 4S.B3 ELISA,WB,ICFC
Alexa Fluor® 488 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 647 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 700 anti-human IFN-γ 4S.B3 ICFC
Pacific Blue™ anti-human IFN-γ 4S.B3 ICFC
PerCP/Cyanine5.5 anti-human IFN-γ 4S.B3 ICFC
APC/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
PE/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 421™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 570™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 605™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 650™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 711™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 785™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 510™ anti-human IFN-γ 4S.B3 ICFC
PE/Dazzle™ 594 anti-human IFN-γ 4S.B3 ICFC
APC/Fire™ 750 anti-human IFN-γ 4S.B3 ICFC
PerCP anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 750™ anti-human IFN-γ 4S.B3 ICFC
KIRAVIA Blue 520™ anti-human IFN-γ Antibody 4S.B3 ICFC
Spark NIR™ 685 anti-human IFN-γ Antibody 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
Spark Blue™ 515 anti-human IFN-γ 4S.B3 ICFC
Spark UV™ 387 anti-human IFN-γ 4S.B3 ICFC
Spark PLUS UV395™ anti-human IFN-γ 4S.B3 ICFC
PE anti-human IFN-γ 4S.B3 ICFC
GMP APC anti-human IFN-γ 4S.B3 ICFC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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