Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse mammary gland epithelial cell line NMuMG

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴ and immunofluorescent staining^2,3.

Application References

(PubMed link indicates BioLegend citation)

1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)

Product Citations

1. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
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5. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
6. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
7. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
8. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
9. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
10. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
11. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
12. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
13. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
14. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
15. Huang L, et al. 2015. J Immunol. 194:283. PubMed
16. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
17. Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
18. Duan L, et al. 2021. Immunity. 54:2256. PubMed
19. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
20. Ise W, et al. 2018. Immunity. 48:702. PubMed
21. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
22. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
23. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
24. Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
25. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
26. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
27. , et al. 2021. Eur J Immunol. 51:2708. PubMed
28. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
29. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
30. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
31. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
32. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
33. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
34. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
35. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
36. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
37. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
38. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
39. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
40. Viant C, et al. 2020. Cell. 183:1298. PubMed
41. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
42. Nascimento DC, et al. 2021. Immunity. :. PubMed
43. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
44. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
45. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
46. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed

RRID

AB_11204257 (BioLegend Cat. No. 142507)
AB_11204257 (BioLegend Cat. No. 142523)
AB_11204257 (BioLegend Cat. No. 142508)

Antigen Details

Structure

Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD

Function

Cell adhesion, cell motility, cell migration, cell proliferation

Cell Type

B cells

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID

20969 View all products for this Gene ID

UniProt

View information about CD138 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis