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PE anti-mouse CD262 (DR5, TRAIL-R2) Antibody

Pricing & Availability
Clone
MD5-1 (See other available formats)
Regulatory Status
RUO
Other Names
DR5, TRAIL-R2, TNFRS10B, TRICK2, KILLER
Isotype
Armenian Hamster IgG
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Product Citations
publications
MD5-1_PE_090707
Mouse DR5 transfected cells stained with MD5-1 PE
  • MD5-1_PE_090707
    Mouse DR5 transfected cells stained with MD5-1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
119905 50 µg 134 CHF
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119906 200 µg 408 CHF
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Description

CD262 is a 55 kD protein also known as DR5, TRAIL-R2, Apo-2, TRICK2, and KILLER. DR5 is member 10B of the TNF receptor superfamily (TNFRSF10B), widely expressed in normal tissues and some tumor cell lines. The DR5 receptor binds to TRAIL and interacts with the cytoplasmic proteins TRADD and RIP. TRAIL binding to DR5 activates NF- κB and induces apoptosis. DR5 is induced by wild-type p53.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse DR5-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo induction of cytotoxicity2,3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 119909).

Application References

(PubMed link indicates BioLegend citation)
  1. Hayakawa Y, et al. 2004. J. Immunol. 172:123. (FC)
  2. Takeda K, et al. 2004. J. Exp. Med. 199:437. (Cyt)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Cyt)
  4. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
  5. Grisendi G, et al. 2010. Cancer Res. 70:3718. PubMed
Product Citations
  1. Iannello A, et al. 2009. J Virol. 83:5999. PubMed
  2. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed
  3. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  4. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  5. West A, et al. 2016. Oncogenesis . 5: e216. PubMed
  6. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  7. Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
  8. Grisendi G, et al. 2010. Cancer Res. 70:3718. PubMed
  9. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  10. Young A, et al. 2011. Bone. 48:857. PubMed
RRID
AB_2205065 (BioLegend Cat. No. 119905)
AB_2205065 (BioLegend Cat. No. 119906)

Antigen Details

Structure
Type I transmembrane protein, TNF receptor superfamily member, homotrimer, predicted molecular weight 55 kD
Distribution

Widely expressed in normal tissues and some tumor cell lines. Expression regulated by the tumor suppressor p53

Function
Binds to TRAIL, activates NF- ?B, induces apoptosis. May be involved in the regulation of tumor metastases.
Ligand/Receptor
TRAIL (CD253); cytoplasmic domain interacts with TRADD and RIP
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Wu GS, et al. 1999. Cancer Res. 59:2770.

Gene ID
21933 View all products for this Gene ID
UniProt
View information about CD262 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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