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PE/Cyanine7 anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
4B10_PECyanine7_T-Bet_Antibody_1_041124
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with T-bet (clone 4B10) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • 4B10_PECyanine7_T-Bet_Antibody_1_041124
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with T-bet (clone 4B10) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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644823 25 µg 171 CHF
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644824 100 µg 429 CHF
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  3. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  4. Jung S, et al. 2022. Cell Rep. 40:111138. PubMed
  5. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  6. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  7. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  8. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  9. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  10. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  11. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  12. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  13. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  14. Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
  15. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  16. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  17. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  18. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  19. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  20. Hong MJ, et al. 2018. Mucosal Immunol. 11:894. PubMed
  21. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  22. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  23. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  24. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  25. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  26. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  27. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  28. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  29. Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed
  30. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  31. Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
  32. Sjaastad FV, et al. 2020. Front Immunol. 11:1786. PubMed
  33. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  34. Hernndez DC, et al. 2021. Immunity. :. PubMed
  35. Joubert IA, et al. 2020. Vaccine. 38:1015. PubMed
  36. Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
  37. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
  38. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  39. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  40. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  41. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
  42. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_2561760 (BioLegend Cat. No. 644823)
AB_2561760 (BioLegend Cat. No. 644824)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org
Go To Top Version: 4    Revision Date: 02.17.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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