Loading...

Purified anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_Pure_021606
C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC
  • 17A2_Pure_021606
    C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC
  • 17A2_Pure_CD3_Antibody_2_102418
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified CD3 (clone 17A2) and Alexa Fluor® 488 anti-mouse/human CD45R/B220 antibody (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (clone MRG2b-85) (red) for two hours at room temperature. The image was captured by 10X objective.
  • 17A2_Pure_CD3_Antibody_3_102418
    Fresh, frozen mouse spleen was stained with purified CD3 clone 17A2 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Compare all formats
Cat # Size Price Quantity Check Availability Save
100201 50 µg 30 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100202 500 µg 94 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Bernstein DL, et al. 2020. Brain Behav Immun. 87:543. PubMed
  4. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  5. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
  6. Li TT, et al. 2021. BMC Immunol. 22:55. PubMed
  7. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  8. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  9. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  10. Campisi L, et al. 2022. Nature. 606:945. PubMed
  11. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  12. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  13. Zhao J, et al. 2022. J Clin Invest. 132: . PubMed
  14. Glaubitz J, et al. 2023. Gut. :. PubMed
  15. Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed
  16. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  17. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  18. Mammadli M, et al. 2023. Int J Mol Sci. 24:. PubMed
  19. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  20. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
  21. Hamada T, et al. 2008. J Immunol. 180:1843. PubMed
  22. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  23. Kulkarni NN, et al. 2020. J Invest Dermatol. 140:645. PubMed
  24. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  25. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  26. Zhao J, et al. 2021. JCI Insight. 6:. PubMed
  27. Bergstrom K, et al. 2008. Infect Immun. 76:796. PubMed
  28. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  29. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  30. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  31. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  32. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  33. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  34. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  35. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  36. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  37. Haque M, et al. 2021. Viruses. 13:. PubMed
  38. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  39. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  40. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  41. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  42. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  43. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  44. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  45. Kerdidani D, et al. 2019. Nat Commun. 10:1405. PubMed
  46. Hayashi Y, et al. 2017. J Cancer. 8:2478. PubMed
  47. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  48. Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed
  49. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  50. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  51. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  52. Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed
  53. Child KM et al. 2018. The Journal of Neuroscience. 38(31):6806-6824 . PubMed
  54. Recino A, et al. 2019. Gene Ther. 26:40. PubMed
  55. Yoshihara T, et al. 2020. Gut Microbes. 11:1385. PubMed
  56. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  57. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  58. Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed
  59. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  60. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  61. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  62. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  63. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  64. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  65. Kopec A, et al. 2014. Toxicol Sci. 141:515. PubMed
  66. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  67. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  68. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  69. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  70. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  71. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  72. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  73. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  74. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  75. You X, et al. 2022. Front Genet. 12:790990. PubMed
  76. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  77. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  78. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  79. Karhadkar TR, et al. 2020. bioRxiv. . PubMed
  80. Gilfillan CB, et al. 2020. Eur J Immunol. 50:505. PubMed
  81. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  82. Fitzpatrick E, et al. 2017. PLoS One. 10.1371/journal.pone.0169273. PubMed
  83. Karhadkar TR, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 318:L165. PubMed
  84. Sugita S, et al. 2017. Arthritis Res Ther. 19:176. PubMed
  85. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  86. Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed
  87. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
  88. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  89. Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed
  90. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
  91. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  92. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  93. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  94. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  95. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  96. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  97. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  98. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  99. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  100. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  101. Gaspar M, et al. 2020. Cancer Immunol Res. 0.875694444. PubMed
  102. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  103. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  104. Tilley S, et al. 2007. J Immunol . 178:3208. PubMed
  105. Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed
  106. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  107. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  108. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  109. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  110. He Y, et al. 2021. eLife. 10:00. PubMed
  111. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  112. Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed
  113. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  114. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  115. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  116. Gonzales C, et al. 2016. PLoS One. 11:e0164557. PubMed
  117. Rastelli D, et al. 2021. J Clin Invest. :e150789. PubMed
  118. Moretti FA, et al. 2021. Sci Rep. 11:23221. PubMed
  119. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  120. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  121. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  122. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  123. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  124. Yadav A, et al. 2009. Am J Physiol Renal Physiol. 298:734. PubMed
  125. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  126. Egaña–Gorroño L, et al. 2019. Atherosclerosis. 289:184. PubMed
  127. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  128. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
RRID
AB_312658 (BioLegend Cat. No. 100201)
AB_312658 (BioLegend Cat. No. 100202)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 01.04.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account