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Purified anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X54-57.1_1_PE_062410
C57BL/6 bone marrow cells stained with CD11b FITC and purified X54-5/7.1 conjugated with PE
  • X54-57.1_1_PE_062410
    C57BL/6 bone marrow cells stained with CD11b FITC and purified X54-5/7.1 conjugated with PE
  • X54-57.1_2_PE_062410
    C57BL/6 bone marrow cells stained with CD11b FITC and mouse IgG1 PE isotype control
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139301 50 µg 71 CHF
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139302 500 µg 323 CHF
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  2. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  3. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  4. Campisi L, et al. 2022. Nature. 606:945. PubMed
  5. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  6. Melo GD, et al. 2017. Sci Rep. 6.1625. PubMed
  7. Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
  8. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  9. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  10. Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed
  11. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  12. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  13. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  14. Feuerstein R, et al. 2020. Elife. 9:00. PubMed
  15. Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
  16. Ozeri E, et al. 2012. J Immunol. 189:146. PubMed
  17. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  18. Blriot C, et al. 2021. Immunity. :. PubMed
  19. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  20. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  21. Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed
  22. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  23. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
  24. Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed
  25. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  26. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  27. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  28. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_10612757 (BioLegend Cat. No. 139301)
AB_10612757 (BioLegend Cat. No. 139302)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

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Go To Top Version: 2    Revision Date: 12.14.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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