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Purified anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119
C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • TER-119
    C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • Ter-119_pure_Ter119Erythroid_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified TER-119/Erythroid Cells clone TER-119 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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116201 50 µg 42 CHF
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116202 500 µg 138 CHF
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  2. Tremblay M, et al. 2020. Elife. 9: . PubMed
  3. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  4. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  5. Grünewald M, et al. 2021. Science. 373: . PubMed
  6. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  7. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  8. Mahamud MR, et al. 2023. Microcirculation. 30:e12787. PubMed
  9. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  10. Baganha F, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  12. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  13. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  14. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
  15. Chen L, et al. 2019. Nat Commun. 3.553472222. PubMed
  16. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  17. Motoyama S, et al. 2020. Cells. :9. PubMed
  18. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  19. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  20. Blume J, et al. 2019. Eur J Immunol. 49:121. PubMed
  21. Heuser M, et al. 2007. Blood. 110:1639. PubMed
  22. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  23. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  24. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  25. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  26. Brown IK, et al. 2021. PLoS Pathog. 17:e1009602. PubMed
  27. Niaudet C, et al. 2019. Angiogenesis. 1.257638889. PubMed
  28. Tahara N, et al. 2018. Dev Biol. 434:74. PubMed
  29. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  30. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  31. You X, et al. 2022. Front Genet. 12:790990. PubMed
  32. Duong CN, et al. 2020. Arterioscler Thromb Vasc Biol. 40:378. PubMed
  33. Malik N, et al. 2019. Sci Rep. 9:16917. PubMed
  34. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  35. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  36. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  37. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  38. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  39. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  40. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  41. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  42. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  43. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  44. Cheng P, et al. 2008. J Exp Med. 205:2235. PubMed
  45. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  46. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  47. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  48. Gough S, et al. 2014. Cancer Discovery. 4:564. PubMed
RRID
AB_313702 (BioLegend Cat. No. 116201)
AB_313702 (BioLegend Cat. No. 116202)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Go To Top Version: 4    Revision Date: 04.23.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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