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- Clone
- F38-2E2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
PHA-activated human PBMCs (3 days) stained with anti-human CD366 (Tim-3) (clone F38-2E2) Brilliant Violet 750™ (filled histogram) or mouse IgG1, κ Brilliant Violet 750™ isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 345055 | 25 tests | 181€ | ||||
| 345056 | 100 tests | 357€ | ||||
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Tim-3 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
-
Application References
(PubMed link indicates BioLegend citation) -
- Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
- Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
- Klibi J, et al 2009. Blood 113:1957. (FC, Block)
- RRID
-
AB_2892420 (BioLegend Cat. No. 345055)
AB_2892420 (BioLegend Cat. No. 345056)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
- Function
- Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
- Ligand/Receptor
- Galectin-9
- Cell Type
- Dendritic cells, Monocytes, T cells, Th1, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342. - Gene ID
- 84868 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD366
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD366
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD366 on UniProt.org
Related FAQs
Other Formats
View All CD366 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD366 (Tim-3)
Th1 polarized human peripheral blood mononuclear cells stain... 
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PE anti-human CD366 (Tim-3)
Th1-polarized human peripheral blood mononuclear cells were ...
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Brilliant Violet 421™ anti-human CD366 (Tim-3)
Th1-polarized human peripheral blood lymphocytes were staine... -
Ultra-LEAF™ Purified anti-human CD366 (Tim-3)
Th1-polarized human peripheral blood mononuclear cells were ... 
-
APC anti-human CD366 (Tim-3)
Th1-polarized cells from human peripheral blood mononuclear ... -
PE/Cyanine7 anti-human CD366 (Tim-3)
Th1-polarized cells from human peripheral blood mononuclear ... -
PerCP/Cyanine5.5 anti-human CD366 (Tim-3)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 605™ anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
FITC anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Purified anti-human CD366 (Tim-3) (Maxpar® Ready)
Human PBMCs were incubated for 3 days in media alone (top) o... 
-
Brilliant Violet 711™ anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC/Cyanine7 anti-human CD366 (Tim-3)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
Brilliant Violet 785™ anti-human CD366 (Tim-3)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
Brilliant Violet 650™ anti-human CD366 (Tim-3)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
Brilliant Violet 510™ anti-human CD366 (Tim-3)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
PE/Dazzle™ 594 anti-human CD366 (Tim-3)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
GoInVivo™ Purified anti-human CD366 (Tim-3)
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APC/Fire™ 750 anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... 
-
Pacific Blue™ anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... 
-
Biotin anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
TotalSeq™-A0169 anti-human CD366 (Tim-3)
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TotalSeq™-C0169 anti-human CD366 (Tim-3)
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PE/Cyanine5 anti-human CD366 (Tim-3)
PHA-activated human PBMCs (3 days) were stained with anti-hu... -
TotalSeq™-B0169 anti-human CD366 (Tim-3)
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Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody
PHA-activated human PBMCs (3 days) stained with anti-human C... -
TotalSeq™-D0169 anti-human CD366 (Tim-3)
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PE/Fire™ 810 anti-human CD366 (Tim-3)
Human peripheral blood mononuclear cells stimulated (3 days)... -
PE/Fire™ 640 anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood monocytes wer... -
PE/Fire™ 700 anti-human CD366 (Tim-3)
PHA-stimulated (3 days) human peripheral blood lymphocytes w...
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