Loading...

PerCP/Cyanine5.5 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1dot4_PerCPCy55_061909.jpg
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
  • HK1dot4_PerCPCy55_061909.jpg
    C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
128011 25 µg 113€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128012 100 µg 268€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Ballesteros I, et al. 2014. J Vis Exp. 94: 52031. PubMed
  2. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  3. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  4. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  5. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  6. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  7. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  8. Kang H, et al. 2022. Adv Mater. 34:e2106500. PubMed
  9. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  10. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  11. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  12. Schick J, et al. 2023. Elife. 12: . PubMed
  13. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  14. Awida Z, et al. 2022. Int J Mol Sci. 23:. PubMed
  15. Asare-Bediako B, et al. 2022. Cells. 11:. PubMed
  16. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  17. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  18. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  19. Garabuczi É, et al. 2023. Front Immunol. 14:1139204. PubMed
  20. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  21. Park JA, et al. 2023. J Immunother Cancer. 11:. PubMed
  22. Solier S, et al. 2023. Nature. 617:386. PubMed
  23. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  24. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  25. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  26. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  27. Israelow B, et al. 2020. bioRxiv. . PubMed
  28. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  29. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  30. Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
  31. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  32. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  33. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  34. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  35. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  36. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  37. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  38. Huard A, et al. 2021. J Immunol. 206:1058. PubMed
  39. Demars A, et al. 2021. PLoS Pathog. 17:e1009887. PubMed
  40. Yang Y, et al. 2020. Cell Death Discov. 0.293055556. PubMed
  41. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  42. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  43. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  44. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  45. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  46. Han YH, et al. 2021. Science. 373:. PubMed
  47. Zhou Z, et al. 2019. Oxid Med Cell Longev. 2019:1209307. PubMed
  48. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  49. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  50. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  51. McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed
  52. Honarpisheh P, et al. 2020. J Neuroinflammation. 0.9625. PubMed
  53. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  54. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  55. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  56. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  57. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  58. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  59. Horn KJ, et al. 2022. Nat Commun. 13:3321. PubMed
  60. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  61. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  62. Paiva-Oliveira E, et al. 2017. Histochem Cell Biol. 10.1007/s00418-017-1556-6. PubMed
  63. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  64. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  65. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  66. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  67. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  68. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  69. Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed
  70. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  71. Blriot C, et al. 2021. Immunity. :. PubMed
  72. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  73. Tang C, et al. 2021. Theranostics. 11:9791. PubMed
  74. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  75. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  76. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  77. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  78. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
  79. Yang Y, et al. 2020. Cell Death Discov. 6:62. PubMed
  80. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  81. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  82. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  83. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  84. Teo T, et al. 2015. J Virol. 89: 7955-7969. PubMed
  85. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  86. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  87. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  88. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  89. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  90. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  91. Budai Z, et al. 2021. Cells. 10:. PubMed
  92. Klein O, et al. 2019. J Allergy Clin Immunol. 144:1074. PubMed
  93. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  94. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  95. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  96. Nishio H, et al. 2020. Regen Ther. 14:262. PubMed
  97. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  98. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  99. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  100. Yajjala V, et al. 2016. J Immunol. 196: 4196 - 4203. PubMed
  101. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  102. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  103. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  104. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  105. Heuslein J, et al. 2016. Sci Rep. 6: 27029. PubMed
  106. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  107. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  108. Chen X, et al. 2021. Cell Death Dis. 12:1123. PubMed
  109. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  110. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  111. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  112. Ikezu S, et al. 2021. Mol Psychiatry. 26:1808. PubMed
  113. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  114. Valdearcos M et al. 2017. Cell metabolism. 26(1):185-197 . PubMed
  115. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  116. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  117. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  118. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  119. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  120. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  121. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
  122. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  123. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  124. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_1659242 (BioLegend Cat. No. 128011)
AB_1659242 (BioLegend Cat. No. 128012)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6C
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6C
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account