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Spark Blue™ 550 anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK7_SPKBL_550_CD3_Antibody_100319
Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Spark Blue™ 550 (filled histogram). Open histogram represents unstained cells.
  • SK7_SPKBL_550_CD3_Antibody_100319
    Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Spark Blue™ 550 (filled histogram). Open histogram represents unstained cells.
Compare all formats See Spark Blue™ 550 spectral data
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344851 25 tests 113€
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344852 100 tests 296€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Im KW, et al. 2023. NPJ Breast Cancer. 9:22. PubMed
  2. Lee JY, et al. 2023. Cell Rep Med. 101091:4. PubMed
  3. Vanuytsel K, et al. 2022. Cytometry A. 101:903. PubMed
  4. Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed
  5. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  6. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  7. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
RRID
AB_2819984 (BioLegend Cat. No. 344851)
AB_2819984 (BioLegend Cat. No. 344852)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC/Fire™ 750 anti-human CD3 SK7 FC
Biotin anti-human CD3 SK7 FC,ICC
Purified anti-human CD3 SK7 FC,ICC,IHC-F,WB
FITC anti-human CD3 SK7 FC
PE anti-human CD3 SK7 FC
Alexa Fluor® 488 anti-human CD3 SK7 FC
APC anti-human CD3 SK7 FC
PerCP/Cyanine5.5 anti-human CD3 SK7 FC
PerCP anti-human CD3 SK7 FC
PE/Cyanine7 anti-human CD3 SK7 FC
APC/Cyanine7 anti-human CD3 SK7 FC
Alexa Fluor® 700 anti-human CD3 SK7 FC
Pacific Blue™ anti-human CD3 SK7 FC
Alexa Fluor® 647 anti-human CD3 SK7 FC
Brilliant Violet 510™ anti-human CD3 SK7 FC
Brilliant Violet 421™ anti-human CD3 SK7 FC
Brilliant Violet 605™ anti-human CD3 SK7 FC
Brilliant Violet 711™ anti-human CD3 SK7 FC
FITC anti-human CD3 SK7 FC
PE anti-human CD3 SK7 FC
Brilliant Violet 785™ anti-human CD3 SK7 FC
PE/Dazzle™ 594 anti-human CD3 SK7 FC
Brilliant Violet 750™ anti-human CD3 SK7 FC
TotalSeq™-A0049 anti-human CD3 SK7 PG
PerCP/Cyanine5.5 anti-human CD3 SK7 FC
TotalSeq™-C0049 anti-human CD3 SK7 PG
Spark Blue™ 550 anti-human CD3 SK7 FC
PE/Cyanine7 anti-human CD3 SK7 FC
TotalSeq™-B0049 anti-human CD3 SK7 PG
Alexa Fluor® 660 anti-human CD3 SK7 FC
APC/Fire™ 810 anti-human CD3 SK7 FC
Spark NIR™ 685 anti-human CD3 SK7 FC
PE/Fire™ 640 anti-human CD3 SK7 FC
PE/Fire™ 700 anti-human CD3 SK7 FC
APC anti-human CD3 SK7 FC
PerCP anti-human CD3 SK7 FC
APC/Fire™ 750 anti-human CD3 SK7 FC
GMP FITC anti-human CD3 SK7 FC
PE/Cyanine5 anti-human CD3 Antibody SK7 FC
GMP PE anti-human CD3 SK7 FC
GMP APC anti-human CD3 SK7 FC
GMP PerCP/Cyanine5.5 anti-human CD3 SK7 FC
Spark YG™ 593 anti-human CD3 SK7 FC
GMP PerCP anti-human CD3 SK7 FC
Spark Violet™ 500 anti-human CD3 SK7 FC
Brilliant Violet 650™ anti-human CD3 SK7 FC
Spark Violet™ 500 anti-human CD3 SK7 FC
Spark PLUS UV395™ anti-human CD3 SK7 FC
Go To Top Version: 2    Revision Date: 09/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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