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Alexa Fluor® 488 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
a-C068C2_AF488_CD206_Antibody_1_FC_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • a-C068C2_AF488_CD206_Antibody_1_FC_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • b-C068C2_AF488_CD206_Antibody_2_FC_082911
  • c-C068C2_A488_CD206_Antibody_3_120420
    C57BL/6 mouse frozen lymph node section fixed, permeabilized and blocked according to standard immunofluorescence protocol. Tissue stained with CD206 (clone C068C2) Alexa Fluor® 488 (green), CD3 (clone 145-2C11) Alexa Fluor® 647 (red) and B220 (clone RA3-6B2) Alexa Fluor® 594 (blue). See additional supplemental data for detailed information.
Compare all formats See Alexa Fluor® 488 spectral data
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141709 25 µg 104€
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141710 100 µg 235€
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chen G, et al. 2021. J Mol Cell Cardiol. 159:62. PubMed
  2. Zhang Z, et al. 2022. Mol Nutr Food Res. 66:e2200300. PubMed
  3. Wang L, et al. 2022. Cell Rep. 41:111462. PubMed
  4. Liang Z, et al. 2023. Front Immunol. 13:944286. PubMed
  5. Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed
  6. Arora H, et al. 2019. Immunity. 50:418. PubMed
  7. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  8. Mitsui K, et al. 2023. J Neuroinflammation. 20:102. PubMed
  9. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  10. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  11. Weiss–Sadan T, et al. 2019. Cell Physiol Biochem. 53:550. PubMed
  12. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  13. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  14. Elbaz M, et al. 2015. Mol Oncol. 9: 906-919. PubMed
  15. Xu DQ, et al. 2021. Acta Pharmacol Sin. 42:1080. PubMed
  16. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  17. Nasser MW, et al. 2015. Cancer Res . 75: 974-985. PubMed
  18. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  19. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  20. Song L, et al. 2020. Am J Physiol Endocrinol Metab. 318:E1004. PubMed
  21. Hu D, et al. 2020. Am J Physiol Heart Circ Physiol. 318:H1525. PubMed
  22. Wu H, et al. 2019. Autophagy. :1. PubMed
  23. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  24. Jiang W, et al. 2021. J Cell Physiol. 236:7711. PubMed
  25. Lynch T, et al. 2017. J Mol Cell Cardiol. 102:83-93. PubMed
  26. Fields L, et al. 2021. Mol Ther. 29:2554. PubMed
  27. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  28. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  29. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  30. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  31. Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed
  32. Tu W, et al. 2021. Cell Death Dis. 12:882. PubMed
  33. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  34. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  35. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  36. Babazadeh S, et al. 2021. Cell Mol Biol Lett. 26:30. PubMed
  37. Zhang F, et al. 2021. J Immunol Res. 2021:5521051. PubMed
  38. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  39. Molgora M, et al. 2020. Cell. 182:886. PubMed
  40. Muñoz M, et al. 2022. ACS Nano. . PubMed
  41. Xiao M, et al. 2022. Mol Oncol. 16:1026. PubMed
  42. Barnette DN, et al. 2018. JCI Insight. 3. PubMed
  43. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_10900445 (BioLegend Cat. No. 141709)
AB_10900445 (BioLegend Cat. No. 141710)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 7    Revision Date: 12/04/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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