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PE/Cyanine7 anti-mouse CD71 Antibody

Pricing & Availability
Clone
RI7217 (See other available formats)
Regulatory Status
RUO
Other Names
Transferrin receptor, T9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RI7217_PECyanine7_CD71_Antibody_1_022324
C57BL/6 mouse bone marrow cells were stained with TER-119 APC and CD71 (clone RI7217) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right). Data shown was gated on total bone marrow cells.
  • RI7217_PECyanine7_CD71_Antibody_1_022324
    C57BL/6 mouse bone marrow cells were stained with TER-119 APC and CD71 (clone RI7217) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right). Data shown was gated on total bone marrow cells.
Compare all formats See PE/Cyanine7 spectral data
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113811 25 µg 100€
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113812 100 µg 264€
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Description

CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).

This clone may also be known as R17217 or R17 217.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
  2. Grisendi S, et al. 2005. Nature 437:147.
  3. van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
  4. Willhelm BT, et al. 2011. Blood 117:27. PubMed
  5. Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
  6. Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed
Product Citations
  1. Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed
  2. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  3. Karantanos T, et al. 2022. Haematologica. :. PubMed
  4. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  5. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  6. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  7. Downes DJ, et al. 2021. Nat Commun. 0.86875. PubMed
  8. Tan X, et al. 2021. Front Oncol. 11:768222. PubMed
  9. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  10. Pei W, et al. 2020. Cell Stem Cell. 27(3):383-395.e8. PubMed
  11. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  12. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  13. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  14. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  15. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  16. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  17. Ito K, et al. 2014. J Biol Chem. 289:29892. PubMed
  18. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  19. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  20. Karantanos T, et al. 2022. Sci Adv. 8:eabl8952. PubMed
  21. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  22. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  23. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  24. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  25. Oudelaar AM, et al. 2020. Nat Commun. 2.348611111. PubMed
  26. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  27. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
RRID
AB_2203383 (BioLegend Cat. No. 113811)
AB_2203383 (BioLegend Cat. No. 113812)

Antigen Details

Structure
Transferrin receptor and is 95 kD.
Distribution

Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.

Function
Mediates iron uptake.
Ligand/Receptor
Transferrin.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.

Gene ID
22042 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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