Alexa Fluor 488 anti-mouse IL-10 Antibody anti-IL-10

Pricing & Availability

Clone: JES5-16E3 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Isotype: Rat IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

Compare all formats See Alexa Fluor® 488 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

505013

100 µg

212€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli-expressed, recombinant mouse IL-10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes: ELISA or ELISPOT Detection^1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. Nos. 504905 & 504906) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization¹⁴: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry³: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry³.
RRID: AB_493513 (BioLegend Cat. No. 505013)

Antigen Details

Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Other Formats

View All IL-10 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse IL-10	JES5-16E3	ICFC
Biotin anti-mouse IL-10	JES5-16E3	ELISA Detection,ELISPOT Detection,ICFC
FITC anti-mouse IL-10	JES5-16E3	ICFC
PE anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,IHC
Alexa Fluor® 647 anti-mouse IL-10	JES5-16E3	ICFC
PE/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Alexa Fluor® 488 anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 421™ anti-mouse IL-10	JES5-16E3	ICFC
Pacific Blue™ anti-mouse IL-10	JES5-16E3	ICFC
PerCP/Cyanine5.5 anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10 (Maxpar® Ready)	JES5-16E3	ELISA Capture,CyTOF®
Brilliant Violet 605™ anti-mouse IL-10	JES5-16E3	ICFC
PE/Dazzle™ 594 anti-mouse IL-10	JES5-16E3	ICFC
APC/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Ultra-LEAF™ Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC
Brilliant Violet 650™ anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 711™ anti-mouse IL-10	JES5-16E3	ICFC

Certificate of Analysis

Customers Also Purchased

PE/Cyanine7 anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer)

Clone: C15.6

PE anti-mouse IL-6

Clone: MP5-20F3

Recombinant Mouse IL-4 (carrier-free)

APC anti-mouse IL-6

Clone: MP5-20F3

Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.