Pacific Blue anti-mouse IL-10 Antibody anti-IL-10

Pricing & Availability

Clone: JES5-16E3 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Isotype: Rat IgG2b, κ
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A_JES5-16E3_PB_092409 — Th2 polarized C57BL/6 splenocytes stained with JES5-16E3 Pacific Blue™ and CD3 (17A2) PE

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505019

25 µg

118€

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505020

100 µg

278€

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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli-expressed, recombinant mouse IL-10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Violet Laser (405 nm)
Application Notes: ELISA or ELISPOT Detection^1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. Nos. 504905 & 504906) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization¹⁴: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry³: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry³.
RRID: AB_2125094 (BioLegend Cat. No. 505019)
AB_2125094 (BioLegend Cat. No. 505020)

Antigen Details

Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

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Other Formats

View All IL-10 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse IL-10	JES5-16E3	ICFC
Biotin anti-mouse IL-10	JES5-16E3	ELISA Detection,ELISPOT Detection,ICFC
FITC anti-mouse IL-10	JES5-16E3	ICFC
PE anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,IHC
Alexa Fluor® 647 anti-mouse IL-10	JES5-16E3	ICFC
PE/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Alexa Fluor® 488 anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 421™ anti-mouse IL-10	JES5-16E3	ICFC
Pacific Blue™ anti-mouse IL-10	JES5-16E3	ICFC
PerCP/Cyanine5.5 anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10 (Maxpar® Ready)	JES5-16E3	ELISA Capture,CyTOF®
Brilliant Violet 605™ anti-mouse IL-10	JES5-16E3	ICFC
PE/Dazzle™ 594 anti-mouse IL-10	JES5-16E3	ICFC
APC/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Ultra-LEAF™ Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC
Brilliant Violet 650™ anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 711™ anti-mouse IL-10	JES5-16E3	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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