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APC/Cyanine7 anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Regulatory Status
RUO
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM59_APCCyanine7_CD31_Antibody_012119.png
Human peripheral blood granulocytes were stained with CD31 (clone WM59) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • WM59_APCCyanine7_CD31_Antibody_012119.png
    Human peripheral blood granulocytes were stained with CD31 (clone WM59) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
303119 25 tests 113€
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303120 100 tests 249€
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  2. Wu X, et al. 2022. Front Immunol. 13:811551. PubMed
  3. Zarobkiewicz M, et al. 2022. J Clin Med. 11: . PubMed
  4. Flaskamp L, et al. 2022. Cells. 11: . PubMed
  5. Basil MC, et al. 2022. Nature. 604:120. PubMed
  6. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  7. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  8. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  9. Webster K, et al. 2013. Hum Reprod. 28:651. PubMed
  10. Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed
  11. Kathiriya JJ, et al. 2022. Nat Cell Biol. 24:10. PubMed
  12. Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed
  13. Piekarska K, et al. 2022. Nat Commun. 13:856. PubMed
  14. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  15. Spearman AD, et al. 2021. Front Pediatr. 9:679572. PubMed
  16. Wu X, et al. 2018. J Immunol Methods. 455:71. PubMed
  17. Xi H, et al. 2020. Cell Stem Cell. 27(1):158-176. PubMed
  18. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  19. Xu J et al. 2019. Elife. 8 pii: e48191. PubMed
  20. Camunas–Soler J, et al. 2020. Cell Metabolism. 31(5):1017-1031. PubMed
  21. Haynes BA, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2168. PubMed
RRID
AB_10640734 (BioLegend Cat. No. 303119)
AB_10640734 (BioLegend Cat. No. 303120)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 2    Revision Date: 01/21/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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