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APC/Fire™ 810 anti-human CD303 (BDCA-2) Antibody

Pricing & Availability
Clone
201A (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
201A_APCFire810_CD303_Antibody_053024
Human peripheral mononuclear cells were stained with anti-human CD123 (clone 6H6) PE and with anti-human CD303 (BDCA-2) (clone 201A) APC/Fire™ 810 (left) or with mouse IgG2a, κ APC/Fire™ 810 isotype control (right). Data shown is gated on the lymphocyte and monocyte populations.
  • 201A_APCFire810_CD303_Antibody_053024
    Human peripheral mononuclear cells were stained with anti-human CD123 (clone 6H6) PE and with anti-human CD303 (BDCA-2) (clone 201A) APC/Fire™ 810 (left) or with mouse IgG2a, κ APC/Fire™ 810 isotype control (right). Data shown is gated on the lymphocyte and monocyte populations.
Compare all formats See APC/Fire™ 810 spectral data
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354247 25 tests 128€
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354248 100 tests 306€
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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Freeman A, et al. 2014. PLoS One. 9:110928. PubMed
  2. Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
RRID
AB_3662260 (BioLegend Cat. No. 354247)
AB_3662260 (BioLegend Cat. No. 354248)

Antigen Details

Structure
Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution

Plasmacytoid dendritic cells

Function
Antigen capture, inhibition of IFN α/β production
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.

Gene ID
170482 View all products for this Gene ID
UniProt
View information about CD303 on UniProt.org

Other Formats

View All CD303 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD303 (BDCA-2) 201A FC
Purified anti-human CD303 (BDCA-2) 201A FC
PE anti-human CD303 (BDCA-2) 201A FC
FITC anti-human CD303 (BDCA-2) 201A FC
PerCP/Cyanine5.5 anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 421™ anti-human CD303 (BDCA-2) 201A FC
PE/Cyanine7 anti-human CD303 (BDCA-2) 201A FC
Purified anti-human CD303 (BDCA-2) (Maxpar® Ready) 201A FC,CyTOF®
Alexa Fluor® 647 anti-human CD303 (BDCA-2) 201A FC
Biotin anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 785™ anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 605™ anti-human CD303 (BDCA-2) 201A FC
PE/Dazzle™ 594 anti-human CD303 (BDCA-2) 201A FC
Alexa Fluor® 700 anti-human CD303 (BDCA-2) 201A FC
Alexa Fluor® 488 anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 510™ anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 711™ anti-human CD303 (BDCA-2) 201A FC
APC/Fire™ 750 anti-human CD303 (BDCA-2) 201A FC
APC/Cyanine7 anti-human CD303 (BDCA-2) 201A FC
TotalSeq™-A0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-C0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-B0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-D0370 anti-human CD303 (BDCA-2) 201A PG
Spark Blue™ 550 anti-human CD303 (BDCA-2) (Flexi-Fluor™) 201A FC
Spark Red™ 718 anti-human CD303 (BDCA-2) (Flexi-Fluor™) 201A FC
APC/Fire™ 810 anti-human CD303 (BDCA-2) 201A FC
Spark Violet™ 538 anti-human CD303 (BDCA-2) 201A FC
PerCP/Fire™ 806 anti-human CD303 (BDCA-2) 201A FC
Go To Top Version: 1    Revision Date: 05/30/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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