Loading...

Biotin anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RA3-6B2_Biotin_090507
C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE
  • RA3-6B2_Biotin_090507
    C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE
  • RA3-6B2_Biotin_B220_Antibody_2_010620
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of biotin anti-mouse B220 (clone RA3-6B2) and Alexa Fluor® 488 anti-mouse CD29 (clone HMβ1-1) (green) overnight at 4°C, followed by 2.5 µg/ml of Spark YG™ 570 Streptavidin (red) for 2 hours at room temperature. The image was captured by 10X objective.
Compare all formats
Cat # Size Price Quantity Check Availability Save
103203 50 µg 20€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103204 500 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  5. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  6. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  7. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  8. del Rio ML, et al. 2022. Front Immunol. 13:887348. PubMed
  9. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  10. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  11. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  12. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  13. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  14. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  15. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  16. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  17. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  18. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  19. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  20. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  21. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  22. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  23. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  24. Zhou L, et al. 2021. Cell Mol Immunol. 18:698. PubMed
  25. Lidak T, et al. 2021. Int J Mol Sci. 22: . PubMed
  26. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  27. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  28. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  29. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  30. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  31. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  32. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  33. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  34. Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed
  35. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  36. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  37. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
  38. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
  39. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  40. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  41. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  42. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  43. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  44. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  45. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  46. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  47. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  48. Jing Zhou, Jun Jin, Toshihisa Kawai 2016. Sci Rep. 6:39105. PubMed
  49. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  50. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  51. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  52. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  53. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  54. Bian Y, et al. 2017. PLoS Pathog. 13:e1006384. PubMed
  55. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  56. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  57. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  58. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  59. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  60. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  61. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  62. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  63. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  64. Davidson S, et al. 2016. EMBO Mol Med. 8: 1099 - 1112. PubMed
  65. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  66. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  67. Zhang Y, et al. 2020. J Immunol. 204:3248. PubMed
  68. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  69. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  70. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  71. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  72. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  73. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  74. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  75. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  76. Ishisono K, Yabe T, and Kita K 2017. J Nutr Biochem. . 10.1016/j.jnutbio.2017.07.016. PubMed
  77. Dong S, et al. 2021. Nature. 591:117. PubMed
  78. Nishime C, et al. 2015. J Immunol. 195: 1883-1890. PubMed
  79. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  80. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  81. Vural A, et al. 2019. J Immunol. 202:1510. PubMed
  82. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  83. D'Agostino MR, et al. 2022. STAR Protoc. 3:101652. PubMed
  84. Chen M, et al. 2021. Cancer Discov. 11:194. PubMed
  85. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  86. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  87. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  88. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  89. Khanom US, et al. 2019. J Immunol. 203:881. PubMed
  90. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  91. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  92. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  93. Gally F, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:64. PubMed
  94. Aichinger M, et al. 2013. J Exp Med. 210:287. PubMed
  95. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  96. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  97. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  98. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  99. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  100. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  101. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  102. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  103. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  104. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  105. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  106. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  107. Shih F, et al. 2006. J Immunol. 176:3438. PubMed
  108. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  109. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
  110. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  111. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  112. Soukupova M, et al. 2021. Mol Ther Methods Clin Dev. 21:399. PubMed
  113. Haase P, et al. 2020. Eur J Immunol. 50:558. PubMed
  114. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  115. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  116. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  117. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  118. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  119. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
  120. Iwata A, et al. 2017. Nat Immunol. 18:563. PubMed
  121. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  122. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  123. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  124. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  125. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  126. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  127. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  128. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  129. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  130. Wang J, et al. 2020. Cell Stem Cell. 27(1):81-97. PubMed
  131. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  132. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  133. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  134. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  135. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  136. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  137. Shen H, et al. 2020. Sci Rep. 10:19453. PubMed
  138. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  139. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  140. Qin J, et al. 2007. J Immunol. 179:6799. PubMed
  141. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  142. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  143. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  144. Young K, et al. 2021. Cell Stem Cell. . PubMed
  145. Becklund B, et al. 2016. Sci Rep. 6:30842. PubMed
  146. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
RRID
AB_312988 (BioLegend Cat. No. 103203)
AB_312988 (BioLegend Cat. No. 103204)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 3    Revision Date: 01/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account