PerCP/Fire™ 780 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2

Clone: RA3-6B2 (See other available formats)
Regulatory Status: RUO
Other Names: B220
Isotype: Rat IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

RA3-6B2_PerCP_Fire780_CD45R_Antibody_1_102324 — C57BL/6 splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse/human CD45R/B220 (clone RA3-6B2) PerCP/FireTM 780 (left) or rat IgG2a, κ PerCP/FireTM 780 isotype control (right).

Compare all formats See PerCP/Fire™ 780 spectral data

Cat #	Size	Price	Quantity Check Availability			Save
103213	25 µg	68€

Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Human
Reported Reactivity: Cat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.

Kishihara K, et al. 1993. Cell 74:143.

Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt: View information about CD45R on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220	RA3-6B2	FC
Biotin anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
FITC anti-mouse/human CD45R/B220	RA3-6B2	FC
PE anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Cyanine5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)	RA3-6B2	FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 750 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Spark Blue™ 550 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark NIR™ 685 anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
PE/Fire™ 640 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Fire™ 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Violet™ 538 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 581 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 570 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Red™ 718 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark UV™ 387 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220	RA3-6B2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/23/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.