PE anti-mouse CD138 (Syndecan-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse mammary gland epithelial cell line NMuMG

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴ and immunofluorescent staining^2,3.

Application References

(PubMed link indicates BioLegend citation)

1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)

Product Citations

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18. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
19. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
20. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
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29. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
30. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
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33. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
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RRID

AB_10916119 (BioLegend Cat. No. 142503)
AB_10916119 (BioLegend Cat. No. 142504)

Antigen Details

Structure

Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD

Function

Cell adhesion, cell motility, cell migration, cell proliferation

Cell Type

B cells

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID

20969 View all products for this Gene ID

UniProt

View information about CD138 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis