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PerCP/Cyanine5.5 anti-mouse CD49d Antibody

Pricing & Availability
Clone
R1-2 (See other available formats)
Regulatory Status
RUO
Other Names
α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
R1-2_PerCPCy5.5_CD49d_Antibody_FC_061914.jpg
C57BL/6 mouse splenocytes were stained with CD49d (clone R1-2) PerCP/Cyanine5.5 or rat IgG2b PerCP/Cyanine5.5 isotype control.
  • R1-2_PerCPCy5.5_CD49d_Antibody_FC_061914.jpg
    C57BL/6 mouse splenocytes were stained with CD49d (clone R1-2) PerCP/Cyanine5.5 or rat IgG2b PerCP/Cyanine5.5 isotype control.
Compare all formats See PerCP/Cyanine5.5 spectral data
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103619 25 µg 118€
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103620 100 µg 287€
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Description

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
AKR/Cum mouse spontaneous T lymphoma line TK1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry of frozen sections5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103629 and 103630).

Application References

(PubMed link indicates BioLegend citation)
  1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
  2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
  3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
  5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Product Citations
  1. Ritzel R, et al. 2016. J Immunol. 196: 3318-3330. PubMed
  2. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  3. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  4. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  5. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
RRID
AB_2563701 (BioLegend Cat. No. 103619)
AB_2563701 (BioLegend Cat. No. 103620)

Antigen Details

Structure
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution

T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells

Function
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.

Gene ID
16401 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 06/20/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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