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PhosphoPair ERK1/2 (Thr202/Tyr204) Antibody Set

Pricing & Availability
Clone
6B8B69; W15133B;
Regulatory Status
RUO
Other Names
Extracellular Signal-Regulated Kinase 1, Extracellular Signal-Regulated Kinase 2, Mitogen-Activated Protein Kinase 3, MAPK3, p44, p42, p44-ERK1, p44-ERK2, p44-MAPK,, p42-MAPK
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Whole cell extracts (15 µg protein) from serum-starved Jurkat cells untreated (-) or treated (+) with 190 nM PMA for 15 minutes were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERK1/2 Phospho (Thr202/Tyr204) antibody (clone 6B8B69) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-ERK1/2 antibody (clone W15133B) was used as a pan ERK1/2 loading control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • PhosphoPair_ERK1-2_Antibody_Set_081120.png
    Whole cell extracts (15 µg protein) from serum-starved Jurkat cells untreated (-) or treated (+) with 190 nM PMA for 15 minutes were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERK1/2 Phospho (Thr202/Tyr204) antibody (clone 6B8B69) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-ERK1/2 antibody (clone W15133B) was used as a pan ERK1/2 loading control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-ERK1/2 Antibody WB, ICC W15133B 25 μg Human, Mouse Rat IgG1, κ
Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody WB 6B8B69 25 μg Human, Mouse Mouse IgG2a, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human, Mouse
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation
The antibodies were purified by affinity chromatography.
Storage & Handling
These antibody solutions should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone W15133B: 0.5 - 2.0 µg/mL
Clone 6B8B69: 0.1 - 1.0 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Antigen Details

Structure
ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD.
Distribution

Ubiquitously expressed/Nucleus and cytosol

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204, and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 
4. Roux PP and Blenis J. 2004. Microbiol. Mol. Biol. Rev. 68:320. 
5. Torii S, et al. 2004. J. Biochem. 136:557. 
6. Clark EA and Haynes RO. 1996. J. Biol. Chem. 271:14814.

Gene ID
5595 View all products for this Gene ID 5594 View all products for this Gene ID
UniProt
View information about ERK1/2 on UniProt.org

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Go To Top Version: 1    Revision Date: 08/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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