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Purified anti-CD49e (Integrin α5) Antibody

Pricing & Availability
Clone
P1D6 (See other available formats)
Regulatory Status
RUO
Other Names
FNRA, VLA5A, Integrin alpha-5, VLA-5, integrin alpha-F, CD49 antigen-like family member E, fibronectin receptor subunit alpha, very late activation protein 5, alpha subunit
Isotype
Mouse IgG3
Ave. Rating
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Product Citations
publications
P1D6_Pure_CD49E_IntegrinAlpha5_Antibody_040516
Human peripheral blood lymphocytes were stained with purified CD49e (Integrin α5) (clone P1D6, filled histogram) or purified mouse IgG3, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • P1D6_Pure_CD49E_IntegrinAlpha5_Antibody_040516
    Human peripheral blood lymphocytes were stained with purified CD49e (Integrin α5) (clone P1D6, filled histogram) or purified mouse IgG3, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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921704 100 µg 100€
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Description

CD49e is a type I integral membrane glycoprotein, known as α5 integrin, or VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronectin and neural adhesion molecule L1. It mediates monocytes migration, it is involved in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1D6 monoclonal antibody was generated against human HT1080 fibrosarcoma cells and reacts with the α5 integrin subunit.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for blocking and functional assays (contact our custom solutions team).

This antibody binds to the cytoplasmic surface of native and denatured muscle AChR α1 subunits, within amino acids 371-386.

P1D6 interferes with binding of integrin-bearing cells to fibronectin but not collagen, laminin 1 or vitronectin coated surfaces.

This antibody neutralizes binding of α5β1 to the central cell adhesion domain of fibronectin, which is located within a 80 kD tryptic fragment.

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA, et al. 1992. J. Cell. Biol. 116(2):489.
  2. Wayner EA, et al. 1987. J. Cell. Biol. 105:1873. (Block)
  3. Wayner EA, et al. 1989. J. Cell. Biol. 109(3):1321.
RRID
AB_2616796 (BioLegend Cat. No. 921704)

Antigen Details

Structure
Integrin α5 is a 115 kD protein.
Distribution
Plasma membrane.
Function
CD49e (Integrin α5) is a component of VLA-5 receptor. It is a cell surface adhesion receptor mediating cell-adhesion to extra cellular matrix or to other cells through heterodimerization and connecting to the cytoskeleton and various signaling molecules within the cells. Complexing with ITGB1 in fibronectin receptor.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
3678 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org
Go To Top Version: 0    Revision Date: 04/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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