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Purified anti-human/mouse Integrin β7 Antibody

Pricing & Availability
Clone
FIB504 (See other available formats)
Regulatory Status
RUO
Workshop
VI 6T-101, VI A024
Other Names
β7 Integrin, integrin βp, ITGB7
Isotype
Rat IgG2a, κ
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Product Citations
publications
FIB504_Purified_073007
Human peripheral blood lymphocytes stained with purified FIB504, followed by anti-mouse IgG FITC
  • FIB504_Purified_073007
    Human peripheral blood lymphocytes stained with purified FIB504, followed by anti-mouse IgG FITC
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321202 100 µg 92€
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Description

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,αIEL/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TK1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).

Application References

(PubMed link indicates BioLegend citation)
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185.
  3. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
  4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
  5. Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  2. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  3. Tan X, et al. 2011. J Virol. 85:8316. PubMed
  4. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  5. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  6. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_571975 (BioLegend Cat. No. 321202)

Antigen Details

Structure
Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution

CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes

Function
Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor
CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.

Gene ID
16421 View all products for this Gene ID 3695 View all products for this Gene ID
UniProt
View information about Integrin beta7 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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