- Clone
- OX-90 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- OX-2, MRC
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![OX-90_PURE_CD200_Antibody_101921 OX-90_PURE_CD200_Antibody_101921](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/OX-90_PURE_CD200_Antibody_101921.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then, the tissue was stained with 10 µg/mL of purified anti-mouse CD200 (OX2) Antibody (clone OX-90) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-rat secondary antibody (clone Poly4054) Alexa Fluor® 594 (red) and CD3e (clone 500A2) Alexa Fluor® 647 (green)for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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123801 | 50 µg | 52€ | ||||
123802 | 500 µg | 210€ |
CD200 (OX-2 antigen) is a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 a highly conserved type I membrane glycoprotein that is expressed on a variety of cell types including thymocytes, some T cells, endothelial and follicular dendritc cells, B cells, and brain tissue (neurons); but not on NK cells, granulocytes, monocytes, or macrophages. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. CD200 is the ligand for CD200 receptor (CD200R). The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or downregulation of myeloid cell activity. Blocking of CD200/CD200R interactions decreases myeloid cell inhibitory thresholds which results in enhanced immune activation.
Product DetailsProduct Details
- Verified Reactivity
- mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble fusion protein of the extracellular region of mouse OX-2 antigen with domains 3 and 4 of rat CD4 fusion protein.
- Formulation
- Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_1236496 (BioLegend Cat. No. 123801)
AB_1236498 (BioLegend Cat. No. 123802)
Antigen Details
- Structure
- Immunoglobulin (Ig) superfamily
- Distribution
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Thymocytes, some T cells, endothelial, B cells
- Function
- Co-stimulatory factor in T cell proliferation
- Ligand/Receptor
- CD200R
- Antigen References
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1. Hoek RM, et al. 2000. Science 290:1768.
2. Gorczynski R, et al. 2004. J. of Immunol. 172:7744.
3. Gorczynski L, et al. 1999. J. Immunol. 162:774.
4. Rosenblum MD, et al. 2004. Blood 103:2691.
5. Zhang S, et al. 2004. J. of Immunol. 173:6786.
6. Barclay AN, et al. 2002. Trends Immunol. 23:285. - Gene ID
- 4345 View all products for this Gene ID
- UniProt
- View information about CD200 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD200 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD200 (OX2) | OX-90 | FC,IHC-F |
PE anti-mouse CD200 (OX2) | OX-90 | FC |
APC anti-mouse CD200 (OX2) | OX-90 | FC |
TotalSeq™-A0079 anti-mouse CD200 (OX2) | OX-90 | PG |
Alexa Fluor® 647 anti-mouse CD200 (OX2) | OX-90 | FC,IHC-F |
Alexa Fluor® 594 anti-mouse CD200 (OX2) | OX-90 | IHC-F |
PE/Dazzle™ 594 anti-mouse CD200 (OX2) | OX-90 | FC |
PE/Cyanine7 anti-mouse CD200 (OX2) | OX-90 | FC |
TotalSeq™-B0079 anti-mouse CD200 (OX2) | OX-90 | PG |
TotalSeq™-C0079 anti-mouse CD200 (OX2) | OX-90 | PG |
Brilliant Violet 421™ anti-mouse CD200 (OX2) | OX-90 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD200 (OX2)
C57BL/6 frozen spleen section was fixed with 4% paraformalde... -
PE anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
APC anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
TotalSeq™-A0079 anti-mouse CD200 (OX2)
-
Alexa Fluor® 647 anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with CD19 Brilliant V... C57 frozen spleen section was fixed with 4% paraformaldehyde... -
Alexa Fluor® 594 anti-mouse CD200 (OX2)
C57BL/6 frozen spleen section was fixed with 4% paraformalde... -
PE/Dazzle™ 594 anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
PE/Cyanine7 anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
TotalSeq™-B0079 anti-mouse CD200 (OX2)
-
TotalSeq™-C0079 anti-mouse CD200 (OX2)
-
Brilliant Violet 421™ anti-mouse CD200 (OX2)
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
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