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Purified anti-mouse CD49d Antibody

Pricing & Availability
Clone
9C10 (MFR4.B) (See other available formats)
Regulatory Status
RUO
Other Names
α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
9C10_Purified_090507
C57BL/6 mouse splenocytes stained with purified 9C10, followed by anti-rat IgG FITC
  • 9C10_Purified_090507
    C57BL/6 mouse splenocytes stained with purified 9C10, followed by anti-rat IgG FITC
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103701 50 µg 67€
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Description

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
(C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  4. Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations
  1. Jia W, et al. 2005. Blood. 106:3854. PubMed
  2. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  3. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  4. Lelouard H, et al. 2012. Gastroenterology. 142:592. PubMed
  5. Zhao D, et al. 2021. Cancers (Basel). 13: . PubMed
RRID
AB_313042 (BioLegend Cat. No. 103701)

Antigen Details

Structure
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution

T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells

Function
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.

Gene ID
16401 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD49d Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD49d 9C10 (MFR4.B) FC
PE anti-mouse CD49d 9C10 (MFR4.B) FC
Purified anti-mouse CD49d 9C10 (MFR4.B) FC,Block,IHC-F
Ultra-LEAF™ Purified anti-mouse CD49d 9C10 (MFR4.B) FC,Block,IHC-F
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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