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Purified anti-rat CD11a Antibody

Pricing & Availability
Clone
WT.1 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-1 α chain, αL Integrin, ITGAL, Ly-15, Ly-21
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
WT-1_PURE_CD11a_FC_080318
Rat splenocytes were stained with purified anti-rat CD11a (clone WT.1; filled histogram) or mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • WT-1_PURE_CD11a_FC_080318
    Rat splenocytes were stained with purified anti-rat CD11a (clone WT.1; filled histogram) or mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
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201902 100 µg 84€
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Description

CD11a is a 160-170 kD glycoprotein also known as αL integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β2 integrin (CD18). The LFA-1 (CD11a/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC)
  2. Springer TA. 1994. Cell 76:301. (IHC)
  3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP, Block)
  4. Devine L, et al. 1996. Immunol. 88:456. (Block)
  5. Ohta Y, et al. 1998. Transplantation 65:1094. (Block)
  6. Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)
Product Citations
  1. Moran J, et al. 2023. Front Immunol. 1235675:14. PubMed
RRID
AB_2749874 (BioLegend Cat. No. 201902)

Antigen Details

Structure
160-170 kD glycoprotein
Distribution

Leukocytes including lymphocytes, monocytes/macrophages, and granulocytes.

Function
Mediates intercellular adhesion and cellular activation.
Interaction
CD18
Ligand/Receptor
CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3)
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.

Gene ID
308995 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org
Go To Top Version: 1    Revision Date: 08/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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