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Alexa Fluor® 700 anti-human CD11c Antibody

Pricing & Availability
Clone
Bu15 (See other available formats)
Regulatory Status
RUO
Workshop
V S143
Other Names
Integrin αx subunit, ITGAX, CR4, p150
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Bu15_AF700_CD11c_Antibody_FC_082112
Human peripheral blood monocytes were stained with CD11c (clone Bu15) Alexa Fluor® 700 (filled histogram) or mouse IgG1 , κ Alexa Fluor® 700 (open histogram).
  • Bu15_AF700_CD11c_Antibody_FC_082112
    Human peripheral blood monocytes were stained with CD11c (clone Bu15) Alexa Fluor® 700 (filled histogram) or mouse IgG1 , κ Alexa Fluor® 700 (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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337219 25 tests 123€
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337220 100 tests 259€
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Description

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42.
  2. Rezzonico R, et al. 2001. Blood 97:2932.
  3. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Skronska-Wasek W, et al. 2021. Eur Respir J. :2002732. PubMed
  2. Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Durlanik S, et al. 2021. Sci Rep. 11:14849. PubMed
  5. Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed
  6. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  7. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  8. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  9. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_2561503 (BioLegend Cat. No. 337219)
AB_2561503 (BioLegend Cat. No. 337220)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution

Myeloid, dendritic cells, NK cells, B cells and T cell subsets

Function
Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.

Gene ID
3687 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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