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Biotin anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Biotin_080207
C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
  • 6D5_Biotin_080207
    C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
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115503 50 µg 44€
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115504 500 µg 108€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Song R, et al. 2022. Immunology. 165:274. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  3. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  7. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  8. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  9. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  10. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  11. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  12. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  13. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  14. Xu H, et al. 2023. Elife. 12:. PubMed
  15. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  16. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  17. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  18. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  21. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  22. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  23. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  24. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  25. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  26. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  27. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  28. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  29. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  30. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  31. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  32. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  33. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  34. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  35. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  36. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  37. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  38. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  39. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  40. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  41. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  42. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  43. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  44. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  45. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  46. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  47. Hutter K, et al. 2020. FEBS J. . PubMed
  48. Dong S, et al. 2021. Nature. 591:117. PubMed
  49. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  50. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  51. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  52. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  53. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  54. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  55. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  56. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  57. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  58. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  59. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  60. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  61. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  62. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  63. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  64. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  65. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  66. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  67. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  68. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  69. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  70. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  71. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  72. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  73. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  74. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  75. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  76. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  77. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  78. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  79. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  80. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  81. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  82. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_313638 (BioLegend Cat. No. 115503)
AB_313638 (BioLegend Cat. No. 115504)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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