- Clone
- M18/2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- integrin β2, β2 integrin, ITGB2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CTL glycoprotein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IP, WB, Block, Stim, IHC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101417-101422).
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Application References
(PubMed link indicates BioLegend citation) -
- Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
- Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
- Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
- Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
- Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
- Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
- Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
- Product Citations
-
- RRID
-
AB_2832276 (BioLegend Cat. No. 101421)
AB_2832276 (BioLegend Cat. No. 101420)
AB_2832276 (BioLegend Cat. No. 101422)
AB_2832276 (BioLegend Cat. No. 101417)
AB_2832276 (BioLegend Cat. No. 101418)
AB_2832276 (BioLegend Cat. No. 101419)
Antigen Details
- Structure
- Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
- Distribution
-
All leukocytes
- Function
- Adhesion
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571. - Gene ID
- 16414 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD18 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse CD18 | M18/2 | FC |
PE anti-mouse CD18 | M18/2 | FC |
Purified anti-mouse CD18 | M18/2 | FC,IHC,IP,WB |
Alexa Fluor® 647 anti-mouse CD18 | M18/2 | FC,3D IHC |
Alexa Fluor® 594 anti-mouse CD18 | M18/2 | IHC-F,FC,3D IHC |
Ultra-LEAF™ Purified anti-mouse CD18 | M18/2 | FC,IP,WB,Block,Stim,IHC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD18
C57BL/6 mouse splenocytes stained with M18/2 FITC -
PE anti-mouse CD18
C57BL/6 splenocytes stained with M18/2 PE -
Purified anti-mouse CD18
C57BL/6 mouse splenocytes stained with purified M18/2, follo... -
Alexa Fluor® 647 anti-mouse CD18
C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 64... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen tissu... -
Alexa Fluor® 594 anti-mouse CD18
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse splenocytes were stained with CD18 (clone M18/... Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen tissu... -
Ultra-LEAF™ Purified anti-mouse CD18
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ...
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